%0 Generic %A Micera, Alessandra %A Puxeddu, Ilaria %A Omar Balzamino, Bijorn %A Bonini, Stefano %A Levi-Schaffer, Francesca %D 2013 %T Primer schedule. %U https://plos.figshare.com/articles/dataset/_Primer_schedule_/232782 %R 10.1371/journal.pone.0047316.t001 %2 https://ndownloader.figshare.com/files/562293 %K immunology %K genetics and genomics %K ophthalmology %K biotechnology %K neuroscience %K Biochemistry %X

A summary of primer names, for/rev primer sequences (5′ to 3′), PCR product size (amplicons in bps), annealing conditions and Genebank accession numbers of each gene investigated. F: forward primer; R: reverse primer.

a

PCR primers were designed by Primer3 software (genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi) and synthesized by MWG (mwg.com/; Ebersberg, Germany).

b

The RNAi were designed by Invitrogen website (rnaidesigner.invitrogen.com/).

c

Amplification parameters were as follows: 37 cycles of 30 s/94°C, 25s/specific Ta, 30 s/72°C, preceded by 15 m/95°C hot start polymerase activation and followed by fluorescence monitoring during linear transition from 55–90°C, 0.01°C for 0.3 sec and further 5 m/72°C incubation.

%I PLOS ONE