2281410 Matthew J. Blow Matthew J. Blow Tyson A. Clark Tyson A. Clark Chris G. Daum Chris G. Daum Adam M. Deutschbauer Adam M. Deutschbauer Alexey Fomenkov Alexey Fomenkov Roxanne Fries Roxanne Fries Jeff Froula Jeff Froula Dongwan D. Kang Dongwan D. Kang Rex R. Malmstrom Rex R. Malmstrom Richard D. Morgan Richard D. Morgan Janos Posfai Janos Posfai Kanwar Singh Kanwar Singh Axel Visel Axel Visel Kelly Wetmore Kelly Wetmore Zhiying Zhao Zhiying Zhao Edward M. Rubin Edward M. Rubin Jonas Korlach Jonas Korlach Len A. Pennacchio Len A. Pennacchio Richard J. Roberts Richard J. Roberts Examples of candidate regulatory unmethylated sites. Public Library of Science 2016 5C DNA binding specificities Prokaryotes DNA methylation acts Type II MTases DNA methylation show motif specificities 620 DNA Methyltransferases prokaryotic genome regulation IIG III map DNA modifications m 6A m 4C organism DNA methylation systems 2016-02-12 09:22:43 Figure https://plos.figshare.com/articles/figure/Examples_of_candidate_regulatory_unmethylated_sites_/2281410 <p>In all panels bar charts show the extent of DNA methylation (inter-pulse duration ratio) at the candidate regulatory unmethylated site (blue) and, for comparison, at the ten immediately flanking upstream and downstream motif instances (red). The sequence interval covered in each chart varies due to the density of motifs across the respective genomes. <b>A)</b> Unmethylated sites are present upstream of the Hpa operon in four <i>Enterobacteria</i> species. In 3 cases, the unmethylated site is at the orthologous GATC, in <i>S</i>. <i>bongori</i>, the unmethylated site is located ~100bp upstream of the conserved sites. <b>B)</b> Conserved unmethylated site upstream of a PadR transcriptional regulator in <i>Arthrobacter</i> species. <b>C)</b> Cluster of unmethylated sites upstream of transcriptional regulator and sugar degradation operon in <i>Spirochaeta smaragdinae</i>. <b>D)</b> Cluster of non- or weakly-methylated sites throughout a non-ribosomal peptide synthase operon.</p>