10.1021/nn500212h.s001
Tatsiana Y. Rakovich
Tatsiana Y.
Rakovich
Omar K. Mahfoud
Omar K.
Mahfoud
Bashir M. Mohamed
Bashir M.
Mohamed
Adriele Prina-Mello
Adriele
Prina-Mello
Kieran Crosbie-Staunton
Kieran
Crosbie-Staunton
Tina Van Den Broeck
Tina
Van Den Broeck
Line De Kimpe
Line De
Kimpe
Alyona Sukhanova
Alyona
Sukhanova
Daniel Baty
Daniel
Baty
Aliaksandra Rakovich
Aliaksandra
Rakovich
Stefan A. Maier
Stefan A.
Maier
Frauke Alves
Frauke
Alves
Frans Nauwelaers
Frans
Nauwelaers
Igor Nabiev
Igor
Nabiev
Patrick Chames
Patrick
Chames
Yuri Volkov
Yuri
Volkov
Highly Sensitive Single Domain Antibody–Quantum Dot Conjugates for Detection of HER2 Biomarker in Lung and Breast Cancer Cells
American Chemical Society
2014
detection
breast cancer biomarkers
breast cancers
disease onset
dyes Alexa Fluor 488
HER 2 expression
Alexa Fluor 568.
lung cancer cell lines
quantum dots
Breast Cancer CellsDespite
cancer biomarkers
QD
HER 2 Biomarker
2014-06-24 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Highly_Sensitive_Single_Domain_Antibody_Quantum_Dot_Conjugates_for_Detection_of_HER2_Biomarker_in_Lung_and_Breast_Cancer_Cells/2280397
Despite the widespread availability of immunohistochemical and other methodologies for screening and early detection of lung and breast cancer biomarkers, diagnosis of the early stage of cancers can be difficult and prone to error. The identification and validation of early biomarkers specific to lung and breast cancers, which would permit the development of more sensitive methods for detection of early disease onset, is urgently needed. In this paper, ultra-small and bright nanoprobes based on quantum dots (QDs) conjugated to single domain anti-HER2 (human epidermal growth factor receptor 2) antibodies (sdAbs) were applied for immunolabeling of breast and lung cancer cell lines, and their performance was compared to that of anti-HER2 monoclonal antibodies conjugated to conventional organic dyes Alexa Fluor 488 and Alexa Fluor 568. The sdAbs–QD conjugates achieved superior staining in a panel of lung cancer cell lines with differential HER2 expression. This shows their outstanding potential for the development of more sensitive assays for early detection of cancer biomarkers.