10.1021/nn500212h.s001 Tatsiana Y. Rakovich Tatsiana Y. Rakovich Omar K. Mahfoud Omar K. Mahfoud Bashir M. Mohamed Bashir M. Mohamed Adriele Prina-Mello Adriele Prina-Mello Kieran Crosbie-Staunton Kieran Crosbie-Staunton Tina Van Den Broeck Tina Van Den Broeck Line De Kimpe Line De Kimpe Alyona Sukhanova Alyona Sukhanova Daniel Baty Daniel Baty Aliaksandra Rakovich Aliaksandra Rakovich Stefan A. Maier Stefan A. Maier Frauke Alves Frauke Alves Frans Nauwelaers Frans Nauwelaers Igor Nabiev Igor Nabiev Patrick Chames Patrick Chames Yuri Volkov Yuri Volkov Highly Sensitive Single Domain Antibody–Quantum Dot Conjugates for Detection of HER2 Biomarker in Lung and Breast Cancer Cells American Chemical Society 2014 detection breast cancer biomarkers breast cancers disease onset dyes Alexa Fluor 488 HER 2 expression Alexa Fluor 568. lung cancer cell lines quantum dots Breast Cancer CellsDespite cancer biomarkers QD HER 2 Biomarker 2014-06-24 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Highly_Sensitive_Single_Domain_Antibody_Quantum_Dot_Conjugates_for_Detection_of_HER2_Biomarker_in_Lung_and_Breast_Cancer_Cells/2280397 Despite the widespread availability of immunohistochemical and other methodologies for screening and early detection of lung and breast cancer biomarkers, diagnosis of the early stage of cancers can be difficult and prone to error. The identification and validation of early biomarkers specific to lung and breast cancers, which would permit the development of more sensitive methods for detection of early disease onset, is urgently needed. In this paper, ultra-small and bright nanoprobes based on quantum dots (QDs) conjugated to single domain anti-HER2 (human epidermal growth factor receptor 2) antibodies (sdAbs) were applied for immunolabeling of breast and lung cancer cell lines, and their performance was compared to that of anti-HER2 monoclonal antibodies conjugated to conventional organic dyes Alexa Fluor 488 and Alexa Fluor 568. The sdAbs–QD conjugates achieved superior staining in a panel of lung cancer cell lines with differential HER2 expression. This shows their outstanding potential for the development of more sensitive assays for early detection of cancer biomarkers.