%0 Journal Article
%A Charlermroj, Ratthaphol
%A Himananto, Orawan
%A Seepiban, Channarong
%A Kumpoosiri, Mallika
%A Warin, Nuchnard
%A Gajanandana, Oraprapai
%A Elliott, Christopher T.
%A Karoonuthaisiri, Nitsara
%D 2014
%T Antibody Array in a Multiwell Plate Format for the
Sensitive and Multiplexed Detection of Important Plant Pathogens
%U https://acs.figshare.com/articles/journal_contribution/Antibody_Array_in_a_Multiwell_Plate_Format_for_the_Sensitive_and_Multiplexed_Detection_of_Important_Plant_Pathogens/2274154
%R 10.1021/ac501424k.s001
%2 https://ndownloader.figshare.com/files/3910177
%K multiwell plate format
%K detection
%K Multiwell Plate Format
%K Important Plant PathogensThe
%K MYSV
%K antibody array
%K ELISA
%K seed export industry
%K fruit blotch bacterium Acidovorax avenae subsp
%K plant pathogens
%K ng
%K IV
%K virus
%K CFU
%X The
global seed market is considered to be an important industry with
a total value of $10,543 million US dollars in 2012. Because plant
pathogens such as bacteria and viruses cause a significant economic
loss to both producers and exporters, the seed export industry urgently
requires rapid, sensitive, and inexpensive testing for the pathogens
to prevent disease spreading worldwide. This study developed an antibody
array in a multiwell plate format to simultaneously detect four crucial
plant pathogens, namely, a bacterial fruit blotch bacterium Acidovorax avenae subsp. citrulli (Aac),
Chilli veinal mottle virus (ChiVMV, potyvirus), Watermelon silver mottle virus (WSMoV, tospovirus serogroup IV), and Melon yellow spot
virus (MYSV, tospovirus). The capture antibodies specific to the pathogens
were immobilized on each well at preassigned positions by an automatic
microarrayer. The antibodies on the arrays specifically captured the
corresponding pathogens present in the sample extracts. The presence
of pathogens bound on the capture antibodies was subsequently detected
by a cocktail of fluorescently conjugated secondary antibodies. The
limits of detection of the developed antibody array for the detection
of Aac, ChiVMV, WSMoV, and MYSV were 5 × 105 CFU/mL,
30 ng/mL, 1000 ng/mL, and 160 ng/mL, respectively, which were very
similar to those of the conventional ELISA method. The antibody array
in a multiwell plate format accurately detected plant pathogens in
single and multiple detections. Moreover, this format enables easy
handling of the assay at a higher speed of operation.
%I ACS Publications