10.1021/bi501202u.s001
Bruce R. Branchini
Bruce R.
Branchini
Tara L. Southworth
Tara L.
Southworth
Danielle M. Fontaine
Danielle M.
Fontaine
Audrey
L. Davis
Audrey
L.
Davis
Curran E. Behney
Curran E.
Behney
Martha H. Murtiashaw
Martha H.
Murtiashaw
A <i>Photinus pyralis</i> and <i>Luciola
italica</i> Chimeric Firefly Luciferase Produces Enhanced Bioluminescence
American Chemical Society
2014
bioluminescence
chimeric enzyme
Luciola italica luciferase
Luciola italica Chimeric Firefly Luciferase Produces Enhanced BioluminescenceWe report
novel PpyLit chimera
Photinus pyralis luciferase
2014-10-14 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/A_i_Photinus_pyralis_i_and_i_Luciola_italica_i_Chimeric_Firefly_Luciferase_Produces_Enhanced_Bioluminescence/2245288
We report the enhanced bioluminescence
properties of a chimeric
enzyme (PpyLit) that contains the N-domain of recombinant <i>Photinus pyralis</i> luciferase joined to the C-domain of recombinant <i>Luciola italica</i> luciferase. Compared to the <i>P. pyralis</i> enzyme, the novel PpyLit chimera exhibited 1.8-fold enhanced flash-height
specific activity, 2.0-fold enhanced integration-based specific activity,
2.9-fold enhanced catalytic efficiency (<i>k</i><sub>cat</sub>/<i>K</i><sub>m</sub>), and a 1.4-fold greater bioluminescence
quantum yield. The results of this study provide an underlying basis
of this unusual example of a chimeric enzyme with enhanced catalytic
properties that are not simply the sum of the contributions of the
two luciferases.