TY - DATA T1 - Versatile Microfluidic Droplets Array for Bioanalysis PY - 2015/01/14 AU - Shan-Wen Hu AU - Bi-Yi Xu AU - Wei-ke Ye AU - Xing-Hua Xia AU - Hong-Yuan Chen AU - Jing-Juan Xu UR - https://acs.figshare.com/articles/journal_contribution/Versatile_Microfluidic_Droplets_Array_for_Bioanalysis/2216002 DO - 10.1021/am5075216.s001 L4 - https://ndownloader.figshare.com/files/3851575 KW - CdSe quantum dots KW - HL KW - cell death rates KW - droplets arrays KW - Christmas Tree structure KW - CEA KW - Versatile Microfluidic Droplets Array KW - cancer biomarkers carcinoma KW - droplets array formation KW - throughput condition screening KW - biosensing approach KW - PDMS KW - plasma treatment KW - MB KW - chip surface KW - novel method KW - droplets array N2 - We propose a novel method to obtain versatile droplets arrays on a regional hydrophilic chip that is fabricated by PDMS soft lithography and regional plasma treatment. It enables rapid liquid dispensation and droplets array formation just making the chip surface in contact with solution. By combining this chip with a special Christmas Tree structure, the droplets array with concentrations in gradient is generated. It possesses the greatly improved performance of convenience and versatility in bioscreening and biosensing. For example, high throughput condition screening of toxic tests of CdSe quantum dots on HL-60 cells are conducted and cell death rates are successfully counted quickly and efficiently. Furthermore, a rapid biosensing approach for cancer biomarkers carcinoma embryonic antigen (CEA) is developed via magnetic beads (MBs)-based sandwich immunoassay methods. ER -