TY - DATA T1 - Multifunctional Polyurethane Sponge for Polymerase Chain Reaction Enhancement PY - 2015/03/04 AU - Seunghwan Seok AU - Sujeong Shin AU - Tae Jae Lee AU - Jae-Min Jeong AU - MinHo Yang AU - Do Hyun Kim AU - Jung Youn Park AU - Seok Jae Lee AU - Bong Gill Choi AU - Kyoung G. Lee UR - https://acs.figshare.com/articles/journal_contribution/Multifunctional_Polyurethane_Sponge_for_Polymerase_Chain_Reaction_Enhancement/2190292 DO - 10.1021/am508101m.s001 L4 - https://ndownloader.figshare.com/files/3824458 KW - PCR KW - DNA amplification KW - Conventional gene extraction methods KW - enhancement KW - species KW - functionalized PU sponges KW - identification KW - Multifunctional Polyurethane Sponge KW - polymerase chain reaction KW - superhydrophobic PU sponges KW - fabrication KW - Polymerase Chain Reaction EnhancementSelective N2 - Selective filtering of target biomaterials from impurities is an important task in DNA amplification through polymerase chain reaction (PCR) enhancement and gene identification to save endangered animals and marine species. Conventional gene extraction methods require complicated steps, skilled persons, and expensive chemicals and instruments to improve DNA amplification. Herein, we proposed an alternative method for overcoming such challenges by imparting secondary functionality using commercially available polyurethane (PU) sponges and cost-effective fabrication approaches through polydopamine and polysiloxane coatings. The porous, highly flexible, and chemically modified superhydrophilic and superhydrophobic PU sponges allow large surface areas and mechanically stable frames for effective extraction of genomic DNA through selective filtering of fish tissues and oils. Furthermore, these chemically modified PU sponges allow separation of genes and improvement of PCR for DNA amplification for the identification of fish species. The combination of a simple fabrication method and functionalized PU sponges could be a useful platform for PCR enhancement and gene-based identification of species for practical applications. ER -