Directed Multistep Biocatalysis for the Synthesis of the Polyketide Oxytetracycline in Permeabilized Cells of Escherichia coli Steffen Krauser Thomas Hoffmann Elmar Heinzle 10.1021/cs501825u.s001 https://acs.figshare.com/articles/journal_contribution/Directed_Multistep_Biocatalysis_for_the_Synthesis_of_the_Polyketide_Oxytetracycline_in_Permeabilized_Cells_of_Escherichia_coli/2188966 We report on tailored regeneration modules for the essential cofactors ATP and NADPH as well as for SAM supply, adapted to support biocatalytic multistep synthesis of oxytetracycline (OTC) in permeabilized cells of E. coli. ATP is regenerated from ADP and AMP by the use of polyphosphate. The efficiency of this system was proven with permeabilized cells producing flaviolin. NADPH is regenerated from NADP<sup>+</sup> by oxidation of phosphonate. A constant level of 0.7 mg/L of SAM is provided by the ligation of ATP and methionine. OTC is synthesized at a rate of 2.05 ng/(h (g of the combined biocatalyst)), reaching a final concentration of 0.9 mg/L. 2015-03-06 00:00:00 regenerated ATP NADP SAM AMP ADP mg NADPH permeabilized cells Escherichia coliWe report OTC support biocatalytic multistep synthesis