Directed Multistep Biocatalysis for the Synthesis
of the Polyketide Oxytetracycline in Permeabilized Cells of Escherichia coli
Steffen Krauser
Thomas Hoffmann
Elmar Heinzle
10.1021/cs501825u.s001
https://acs.figshare.com/articles/journal_contribution/Directed_Multistep_Biocatalysis_for_the_Synthesis_of_the_Polyketide_Oxytetracycline_in_Permeabilized_Cells_of_Escherichia_coli/2188966
We report on tailored regeneration
modules for the essential cofactors
ATP and NADPH as well as for SAM supply, adapted to support biocatalytic
multistep synthesis of oxytetracycline (OTC) in permeabilized cells
of E. coli. ATP is regenerated from
ADP and AMP by the use of polyphosphate. The efficiency of this system
was proven with permeabilized cells producing flaviolin. NADPH is
regenerated from NADP<sup>+</sup> by oxidation of phosphonate. A constant
level of 0.7 mg/L of SAM is provided by the ligation of ATP and methionine.
OTC is synthesized at a rate of 2.05 ng/(h (g of the combined biocatalyst)),
reaching a final concentration of 0.9 mg/L.
2015-03-06 00:00:00
regenerated
ATP
NADP
SAM
AMP
ADP
mg
NADPH
permeabilized cells
Escherichia coliWe report
OTC
support biocatalytic multistep synthesis