Phthalocyanine
Derivatives Possessing 2‑(Morpholin-4-yl)ethoxy
Groups As Potential Agents for Photodynamic Therapy
Malgorzata Kucinska
Paulina Skupin-Mrugalska
Wojciech Szczolko
Lukasz Sobotta
Mateusz Sciepura
Ewa Tykarska
Marcin Wierzchowski
Anna Teubert
Agnieszka Fedoruk-Wyszomirska
Eliza Wyszko
Maria Gdaniec
Mariusz Kaczmarek
Tomasz Goslinski
Jadwiga Mielcarek
Marek Murias
10.1021/acs.jmedchem.5b00052.s003
https://acs.figshare.com/articles/dataset/Phthalocyanine_Derivatives_Possessing_2_Morpholin_4_yl_ethoxy_Groups_As_Potential_Agents_for_Photodynamic_Therapy/2186716
Three 2-(morpholin-4-yl)ethoxy
substituted phthalocyanines were
synthesized and characterized. Phthalocyanine derivatives revealed
moderate to high quantum yields of singlet oxygen production depending
on the solvent applied (e.g., in DMF ranging from 0.25 to 0.53). Their
photosensitizing potential for photodynamic therapy was investigated
in an in vitro model using cancer cell lines. Biological test results
were found particularly encouraging for the zinc(II) phthalocyanine
derivative possessing two 2-(morpholin-4-yl)ethoxy substituents in
nonperipheral positions. Cells irradiated for 20 min at 2 mW/cm<sup>2</sup> revealed the lowest IC<sub>50</sub> value at 0.25 μM
for prostate cell line (PC3), whereas 1.47 μM was observed for
human malignant melanoma (A375) cells. The cytotoxic activity in nonirradiated
cells of novel phthalocyanine was found to be very low. Moreover,
the cellular uptake, localization, cell cycle, apoptosis through an
ELISA assay, and immunochemistry method were investigated in LNCaP
cells. Our results showed that the tested photosensitizer possesses
very interesting biological activity, depending on experimental conditions.
2015-03-12 00:00:00
Biological test results
0.25 μ M
nonperipheral positions
cytotoxic activity
LNCaP cells
ELISA assay
PC
novel phthalocyanine
cell cycle
nonirradiated cells
DMF
quantum yields
Potential Agents
Phthalocyanine derivatives
1.47 μ M
singlet oxygen production
20 min
cancer cell lines
photodynamic therapy
prostate cell line
IC 50 value
immunochemistry method