10.1021/jf505655b.s001
Wan Hua
Wan
Hua
Huiling Hu
Huiling
Hu
Fang Chen
Fang
Chen
Lin Tang
Lin
Tang
Tong Peng
Tong
Peng
Zhanguo Wang
Zhanguo
Wang
Rapid Isolation and Purification of Phorbol Esters
from Jatropha curcas by High-Speed
Countercurrent Chromatography
American Chemical Society
2015
HPLC
PE
UPLC
JC 1
Jatropha curcas
MS
HSCCC
jatropha factor C 1
UV absorption spectrum
2015-03-18 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Rapid_Isolation_and_Purification_of_Phorbol_Esters_from_Jatropha_curcas_by_High_Speed_Countercurrent_Chromatography/2185426
In
this work, a high-speed countercurrent chromatography (HSCCC)
method was established for the preparation of phorbol esters (PEs)
from Jatropha curcas. <i>n</i>-Hexane–ethyl acetate–methanol–water (1.5:1.5:1.2:0.5,
v/v) was selected as the optimum two-phase solvent system to separate
and purify jatropha factor C<sub>1</sub> (JC1) with a purity of 85.2%,
as determined by HPLC, and to obtain a mixture containing four or
five PEs. Subsequently, continuous semipreparative HPLC was applied
to further purify JC1 (99.8% as determined by HPLC). In addition,
UPLC–PDA and UPLC–MS were established and successfully
used to evaluate the isolated JC1 and PE-rich crude extract. The purity
of JC1 was only 87.8% by UPLC–UV. A peak (a compound highly
similar to JC1) was indentified as the isomer of JC1 by comparing
the characteristic UV absorption and MS spectra. Meanwhile, this strategy
was also applied to analyze the PE-rich crude extract from J. curcas. It is interesting that there may be more
than 15 PEs according to the same quasi-molecular ion peaks, highly
similar sequence-specific fragment ions, and similar UV absorption
spectrum.