TY - DATA T1 - CAR expression is increased in polarized CaLu-3 cells 18 h post-sidestream cigarette smoke (SSS) exposure relative to mainstream cigarette smoke (MSS), or filtered air (SSFA or MSFA) exposure. PY - 2012/11/15 AU - Priyanka Sharma AU - Abimbola O. Kolawole AU - Susan B. Core AU - Adriana E. Kajon AU - Katherine J. D. A. Excoffon UR - https://plos.figshare.com/articles/figure/_CAR_expression_is_increased_in_polarized_CaLu_3_cells_18_h_post_sidestream_cigarette_smoke_SSS_exposure_relative_to_mainstream_cigarette_smoke_MSS_or_filtered_air_SSFA_or_MSFA_exposure_/212298 DO - 10.1371/journal.pone.0049930.g003 L4 - https://ndownloader.figshare.com/files/541813 KW - polarized KW - calu-3 KW - cells KW - 18 KW - post-sidestream KW - filtered N2 - A: Total CAR mRNA (three to four biological replicates per condition measured in duplicate in each qPCR assay; three independent experiments) and B: CAREx8 mRNA (three to four biological replicates per condition measured in duplicate in each qPCR assay; three independent experiments) quantification using quantitative RT-PCR. Mean values of three independent experiments relative to control+SE of the mean. C: Total CAR and D: CAREx8 protein and corresponding β-actin expression by Western blot (representative) and Multi-Guage image analysis (mean values from three independent experiments expressed as a percentage of control+SE of the mean). *p<0.05. ER -