Development of an Aptamer-Based
Sensing Platform for
Metal Ions, Proteins, and Small Molecules through Terminal Deoxynucleotidyl
Transferase Induced G‑Quadruplex Formation
Ka-Ho Leung
Bingyong He
Chao Yang
Chung-Hang Leung
Hui-Min David Wang
Dik-Lung Ma
10.1021/acsami.5b08314.s001
https://acs.figshare.com/articles/journal_contribution/Development_of_an_Aptamer_Based_Sensing_Platform_for_Metal_Ions_Proteins_and_Small_Molecules_through_Terminal_Deoxynucleotidyl_Transferase_Induced_G_Quadruplex_Formation/2113462
We report a label-free, structure-independent
luminescent-sensing
platform for metal ions, proteins, and small molecules utilizing an
Ir(III) complex, terminal deoxynucleotidyl transferase (TdT), and
a structure-folding aptamer. A novel G-quadruplex-selective Ir(III)
complex was identified to detect the nascent G-quadruplex motifs with
an enhanced luminescence response. Unlike most label-free DNA-based
assays reported in the literature, this sensing platform does not
require a specific secondary structure of aptamer, thus greatly simplifying
DNA design. The detection platform was demonstrated by the detection
of K<sup>+</sup> ions, thrombin, and cocaine as representative examples
of metal ions, proteins, and small molecules.
2015-11-04 00:00:00
terminal deoxynucleotidyl transferase
protein
platform
metal ions
DNA
molecule
detection
Ir
aptamer