10.1371/journal.pone.0050272.g006 Dass S. Vinay Dass S. Vinay Seung J. Lee Seung J. Lee Chang H. Kim Chang H. Kim Ho Sik Oh Ho Sik Oh Byoung S. Kwon Byoung S. Kwon Anti-4-1BB pre-activated PDCA-1<sup>+</sup> B cells inhibit in vitro Ig class switching. Public Library of Science 2013 pre-activated cells inhibit vitro ig 2013-02-19 19:56:30 Figure https://plos.figshare.com/articles/figure/_Anti_4_1BB_pre_activated_PDCA_1_B_cells_inhibit_in_vitro_Ig_class_switching_/209443 <p><b>A</b> PDCA-1<sup>+</sup> B cells were sort-purified from spleens of naïve B6 mice and stained as indicated, and flow cytometry was performed. One of four independent experiments is shown (<i>n</i> = 4). <b>B</b> Purified splenic PDCA-1<sup>+</sup> B cells were stimulated with the indicated agonists, after which in vitro Ig class switching was analyzed by flow cytometry. One of four independent experiments is shown (<i>n</i> = 4). <b>C</b> Purified splenic PDCA-1<sup>+</sup> B cells were stimulated with soluble anti-4-1BB (5 µg/ml) and/or IL-7 (100 ng/ml) for 3d then re-stimulated with LPS/IL-4 for 4d, after which in vitro Ig class switching was analyzed by flow cytometry. One of four independent experiments is shown (<i>n</i> = 4).</p>