10.1371/journal.pone.0050272.g006
Dass S. Vinay
Dass
S. Vinay
Seung J. Lee
Seung J.
Lee
Chang H. Kim
Chang H.
Kim
Ho Sik Oh
Ho
Sik Oh
Byoung S. Kwon
Byoung
S. Kwon
Anti-4-1BB pre-activated PDCA-1<sup>+</sup> B cells inhibit in vitro Ig class switching.
Public Library of Science
2013
pre-activated
cells
inhibit
vitro
ig
2013-02-19 19:56:30
Figure
https://plos.figshare.com/articles/figure/_Anti_4_1BB_pre_activated_PDCA_1_B_cells_inhibit_in_vitro_Ig_class_switching_/209443
<p><b>A</b> PDCA-1<sup>+</sup> B cells were sort-purified from spleens of naïve B6 mice and stained as indicated, and flow cytometry was performed. One of four independent experiments is shown (<i>n</i> = 4). <b>B</b> Purified splenic PDCA-1<sup>+</sup> B cells were stimulated with the indicated agonists, after which in vitro Ig class switching was analyzed by flow cytometry. One of four independent experiments is shown (<i>n</i> = 4). <b>C</b> Purified splenic PDCA-1<sup>+</sup> B cells were stimulated with soluble anti-4-1BB (5 µg/ml) and/or IL-7 (100 ng/ml) for 3d then re-stimulated with LPS/IL-4 for 4d, after which in vitro Ig class switching was analyzed by flow cytometry. One of four independent experiments is shown (<i>n</i> = 4).</p>