10.1021/acs.jnatprod.5b00894.s001 Chen Zhang Chen Zhang Shu Wang Shu Wang Ke-Wu Zeng Ke-Wu Zeng Jun Li Jun Li Daneel Ferreira Daneel Ferreira Jordan K. Zjawiony Jordan K. Zjawiony Bing-Yu Liu Bing-Yu Liu Xiao-Yu Guo Xiao-Yu Guo Hong-Wei Jin Hong-Wei Jin Yong Jiang Yong Jiang Peng-Fei Tu Peng-Fei Tu Nitric Oxide Inhibitory Dimeric Sesquiterpenoids from <i>Artemisia rupestris</i> American Chemical Society 2016 BV microglial cycloaddition basis lipopolysaccharide inhibition Stereoselectivity Compound Diel Artemisia rupestris NMR data Artemisia rupestrisTwelve formation configuration dimeric sesquiterpenoids ECD spectra Nitric Oxide Inhibitory Dimeric Sesquiterpenoids IC 50 values MS biocatalyzed 2016-01-22 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Nitric_Oxide_Inhibitory_Dimeric_Sesquiterpenoids_from_i_Artemisia_rupestris_i_/2084644 Twelve new dimeric sesquiterpenoids (<b>1</b>–<b>12</b>) were isolated from the dried whole plants of <i>Artemisia rupestris</i>. Their structures were determined using MS and NMR data, and the absolute configurations were elucidated on the basis of experimental and calculated ECD spectra. Compounds <b>1</b>–<b>9</b> are presumably formed via biocatalyzed [2+2] or [4+2] cycloaddition reactions. Stereoselectivity of the [4+2] Diels–Alder reaction dictated the formation of <i>endo</i>-products. The dimeric sesquiterpenoids exhibited moderate inhibition on NO production stimulated by lipopolysaccharide in BV-2 microglial cells, with IC<sub>50</sub> values in the range 17.0–71.8 μM.