%0 Figure %A Kobuchi, Hirotsugu %A Moriya, Koko %A Ogino, Tetsuya %A Fujita, Hirofumi %A Inoue, Keiji %A Shuin, Taro %A Yasuda, Tatsuji %A Utsumi, Kozo %A Utsumi, Toshihiko %D 2013 %T Regulation of the intra-mitochondrial content of ALA-mediated PpIX by Ko143, a specific inhibitor of ABCG2. %U https://plos.figshare.com/articles/figure/_Regulation_of_the_intra_mitochondrial_content_of_ALA_mediated_PpIX_by_Ko143_a_specific_inhibitor_of_ABCG2_/207431 %R 10.1371/journal.pone.0050082.g004 %2 https://ndownloader.figshare.com/files/536943 %K intra-mitochondrial %K ala-mediated %K ppix %K inhibitor %X

U937 cells and A549 cells were incubated for 3 h with 1 mM ALA in complete medium in the presence or absence of 1 µM Ko143. After incubation, cells were washed with ALA-free medium and mitochondrial and cytosolic fractions were isolated as described in Materials and Methods. The fluorescence spectrum of each fraction was analyzed by a fluorophotometer. A representative histogram of three independent experiments is shown. The levels of PpIX were shown by black line (No treatment), red line (ALA without Ko143), green line (ALA with Ko143), respectively.

%I PLOS ONE