%0 Journal Article
%A Luanpitpong, Sudjit
%A Chen, Michael
%A Knuckles, Travis
%A Wen, Sijin
%A Luo, Juhua
%A Ellis, Emily
%A Hendryx, Michael
%A Rojanasakul, Yon
%D 2015
%T Appalachian
Mountaintop Mining Particulate Matter
Induces Neoplastic Transformation of Human Bronchial Epithelial Cells
and Promotes Tumor Formation
%U https://acs.figshare.com/articles/journal_contribution/Appalachian_Mountaintop_Mining_Particulate_Matter_Induces_Neoplastic_Transformation_of_Human_Bronchial_Epithelial_Cells_and_Promotes_Tumor_Formation/2042955
%R 10.1021/es504263u.s001
%2 https://ndownloader.figshare.com/files/3614271
%K neoplastic transformation
%K results show
%K epithelial cells
%K tumor formation
%K tumor promotion
%K chemical constituent
%K mountaintop coal mining
%K Human Bronchial Epithelial Cells
%K Chronic exposure
%K cell transformation
%K exposure control
%K tumor growth
%K lung cancer incidence
%K Appalachian Mountaintop Mining Particulate Matter Induces Neoplastic Transformation
%K lung carcinoma H 460 cells
%K cell proliferation
%K Promotes Tumor FormationEpidemiological studies
%K PMMTM effects
%K control particle PMCON
%K cell migration
%K Xenograft transplantation
%K lung cells
%K risk assessment
%X Epidemiological studies suggest that
living near mountaintop coal
mining (MTM) activities is one of the contributing factors for high
lung cancer incidence. The purpose of this study was to investigate
the long-term carcinogenic potential of MTM particulate matter (PMMTM) exposure on human bronchial epithelial cells. Our results
show that chronic exposure (3 months) to noncytotoxic, physiological
relevant concentration (1 μg/mL) of PMMTM, but not
control particle PMCON, induced neoplastic transformation,
accelerated cell proliferation, and enhanced cell migration of the
exposed lung cells. Xenograft transplantation of the PMMTM-exposed cells in mice caused no apparent tumor formation, but promoted
tumor growth of human lung carcinoma H460 cells, suggesting the tumor-promoting
effect of PMMTM. Chronic exposure to the main inorganic
chemical constituent of PMMTM, molybdenum but not silica,
similarly induced cell transformation and tumor promotion, suggesting
the contribution of molybdenum, at least in part, in the PMMTM effects. These results provide new evidence for the carcinogenic
potential of PMMTM and support further risk assessment
and implementation of exposure control for PMMTM.
%I ACS Publications