%0 Journal Article
%A Garg, Ashish
%A Xie, Xinqiang
%A Keatinge-Clay, Adrian
%A Khosla, Chaitan
%A Cane, David E.
%D 2015
%T Elucidation
of the Cryptic Epimerase Activity of Redox-Inactive
Ketoreductase Domains from Modular Polyketide Synthases by Tandem
Equilibrium Isotope Exchange
%U https://acs.figshare.com/articles/journal_contribution/Elucidation_of_the_Cryptic_Epimerase_Activity_of_Redox_Inactive_Ketoreductase_Domains_from_Modular_Polyketide_Synthases_by_Tandem_Equilibrium_Isotope_Exchange/2036874
%R 10.1021/ja5056998.s001
%2 https://ndownloader.figshare.com/files/3608184
%K nonepimerizing EryKR 6 domain
%K epimerase activity
%K EryKR 30 domain
%K PKS
%K parent EryKR 1 domain
%K Modular Polyketide Synthases
%K module 3
%K EIX
%K tandem equilibrium isotope exchange
%K polyketide synthases harbor
%K Tandem Equilibrium Isotope ExchangeMany
%K EryKR 10 mutants
%K Cryptic Epimerase Activity
%K NADPH binding site
%K epimerization
%X Many modular polyketide synthases
harbor one or more redox-inactive
domains of unknown function that are highly homologous to ketoreductase
(KR) domains. A newly developed tandem equilibrium isotope exchange
(EIX) assay has now established that such “KR0”
domains catalyze the biosynthetically essential epimerization of transient
(2R)-2-methyl-3-ketoacyl-ACP intermediates to the
corresponding (2S)-2-methyl-3-ketoacyl-ACP diastereomers.
Incubation of [2-2H]-(2R,3S)-2-methyl-3-hydroxypentanoyl-SACP ([2-2H]-3b) with the EryKR30 domain from module 3 of the
6-deoxyerythronolide B synthase, and the redox-active,
nonepimerizing EryKR6 domain and NADP+ resulted in time-
and cofactor-dependent washout of deuterium from 3b,
as a result of EryKR30-catalyzed epimerization of transiently
generated [2-2H]-2-methyl-3-ketopentanoyl-ACP (4). Similar results were obtained with redox-inactive PicKR30 from module 3 of the picromycin synthase. Four redox-inactive mutants
of epimerase-active EryKR1 were engineered by mutagenesis of the NADPH
binding site of this enzyme. Tandem EIX established that these EryKR10 mutants retained the intrinsic epimerase activity of the
parent EryKR1 domain. These results establish the intrinsic epimerase
activity of redox-inactive KR0 domains, rule out any role
for the NADPH cofactor in epimerization, and provide a general experimental
basis for decoupling the epimerase and reductase activities of a large
class of PKS domains.
%I ACS Publications