10.1021/ac500305j.s001
Masanori Kaneko
Masanori
Kaneko
Yoshinori Takano
Yoshinori
Takano
Yoshito Chikaraishi
Yoshito
Chikaraishi
Nanako
O. Ogawa
Nanako
O.
Ogawa
Susumu Asakawa
Susumu
Asakawa
Takeshi Watanabe
Takeshi
Watanabe
Seigo Shima
Seigo
Shima
Martin Krüger
Martin
Krüger
Makoto Matsushita
Makoto
Matsushita
Hiroyuki Kimura
Hiroyuki
Kimura
Naohiko Ohkouchi
Naohiko
Ohkouchi
Quantitative Analysis of Coenzyme F430 in Environmental
Samples: A New Diagnostic Tool for Methanogenesis and Anaerobic Methane
Oxidation
American Chemical Society
2015
geochemical techniques
F 430
paddy soils
Quantitative Analysis
marine sediments
methane oxidation
methane production
nickel hydrocorphinoid
F 430 analysis
F 430 concentrations
detection limit
0.1 femto mol
New Diagnostic Tool
methanogenic archaeal biomass
Anaerobic Methane OxidationCoenzyme F 430
ANME
samples range
Environmental Samples
prosthetic group
methanogenic reaction sequence
Coenzyme F 430
2015-12-17 01:19:01
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Quantitative_Analysis_of_Coenzyme_F430_in_Environmental_Samples_A_New_Diagnostic_Tool_for_Methanogenesis_and_Anaerobic_Methane_Oxidation/2030784
Coenzyme F430 is a nickel hydrocorphinoid
and is the prosthetic
group of methyl–coenzyme M reductase that catalyzes the last
step of the methanogenic reaction sequence and its reversed reaction
for anaerobic methane oxidation by ANME. As such, function-specific
compound analysis has the potential to reveal the microbial distribution
and activity associated with methane production and consumption in
natural environments and, in particular, in deep subsurface sediments
where microbiological and geochemical techniques are restricted. Herein,
we report the development of a technique for high-sensitivity analysis
of F430 in environmental samples, including paddy soils, marine sediments,
microbial mats, and an anaerobic groundwater. The lower detection
limit of F430 analysis by liquid chromatography/mass spectrometry
is 0.1 femto mol, which corresponds to 6 × 10<sup>2</sup> to
1 × 10<sup>4</sup> cells of methanogens. F430 concentrations
in these natural environmental samples range from 63 × 10<sup>–6</sup> to 44 nmol g<sup>–1</sup> and are consistent
with the methanogenic archaeal biomass estimated by microbiological
analyses.