%0 Journal Article
%A Lv, Haitao
%A Hung, Chia S.
%A Henderson, Jeffrey P.
%D 2015
%T Metabolomic Analysis of Siderophore
Cheater Mutants Reveals Metabolic Costs of Expression in Uropathogenic Escherichia coli
%U https://acs.figshare.com/articles/journal_contribution/Metabolomic_Analysis_of_Siderophore_Cheater_Mutants_Reveals_Metabolic_Costs_of_Expression_in_Uropathogenic_i_Escherichia_coli_i_/2029776
%R 10.1021/pr4009749.s001
%2 https://ndownloader.figshare.com/files/3601089
%K metabolomic approach
%K costs influence success
%K Siderophore Cheater Mutants
%K impact
%K Uropathogenic Escherichia coliBacterial siderophores
%K iron acquisition function
%K siderophore biosynthetic genes
%K uropathogen parent strain
%K K 12 strain
%X Bacterial
siderophores are a group of chemically diverse, virulence-associated
secondary metabolites whose expression exerts metabolic costs. A combined
bacterial genetic and metabolomic approach revealed differential metabolomic
impacts associated with biosynthesis of different siderophore structural
families. Despite myriad genetic differences, the metabolome of a
cheater mutant lacking a single set of siderophore biosynthetic genes
more closely approximate that of a non-pathogenic K12 strain than
its isogenic, uropathogen parent strain. Siderophore types associated
with greater metabolomic perturbations are less common among human
isolates, suggesting that metabolic costs influence success in a human
population. Although different siderophores share a common iron acquisition
function, our analysis shows how a metabolomic approach can distinguish
their relative metabolic impacts in E. coli.
%I ACS Publications