%0 Journal Article %A Lv, Haitao %A Hung, Chia S. %A Henderson, Jeffrey P. %D 2015 %T Metabolomic Analysis of Siderophore Cheater Mutants Reveals Metabolic Costs of Expression in Uropathogenic Escherichia coli %U https://acs.figshare.com/articles/journal_contribution/Metabolomic_Analysis_of_Siderophore_Cheater_Mutants_Reveals_Metabolic_Costs_of_Expression_in_Uropathogenic_i_Escherichia_coli_i_/2029776 %R 10.1021/pr4009749.s001 %2 https://ndownloader.figshare.com/files/3601089 %K metabolomic approach %K costs influence success %K Siderophore Cheater Mutants %K impact %K Uropathogenic Escherichia coliBacterial siderophores %K iron acquisition function %K siderophore biosynthetic genes %K uropathogen parent strain %K K 12 strain %X Bacterial siderophores are a group of chemically diverse, virulence-associated secondary metabolites whose expression exerts metabolic costs. A combined bacterial genetic and metabolomic approach revealed differential metabolomic impacts associated with biosynthesis of different siderophore structural families. Despite myriad genetic differences, the metabolome of a cheater mutant lacking a single set of siderophore biosynthetic genes more closely approximate that of a non-pathogenic K12 strain than its isogenic, uropathogen parent strain. Siderophore types associated with greater metabolomic perturbations are less common among human isolates, suggesting that metabolic costs influence success in a human population. Although different siderophores share a common iron acquisition function, our analysis shows how a metabolomic approach can distinguish their relative metabolic impacts in E. coli. %I ACS Publications