10.1371/journal.pone.0050442.g007 Cristian Acosta Cristian Acosta Simon McMullan Simon McMullan Laiche Djouhri Laiche Djouhri Linlin Gao Linlin Gao Roger Watkins Roger Watkins Carol Berry Carol Berry Katherine Dempsey Katherine Dempsey Sally N. Lawson Sally N. Lawson NT3 expression in DRGs 1 and 4 days after Inflammation. Public Library of Science 2012 drgs days 2012-12-07 02:41:27 Figure https://plos.figshare.com/articles/figure/_NT3_expression_in_DRGs_1_and_4_days_after_Inflammation_/199687 <p><b>A</b>: Western blots of pools of 2 DRGs (L4 and L5) from one rat per pool. Upper: NT3 examples from two normal rats, two 1 day CFA rats (ipsilateral DRGs) and two 4 day CFA rats. Lower: the α-tubulin Westerns on the same lane as the NT3 as loading controls. The NT3 has decreased at both 1 and 4 days, both ipsilaterally and contralaterally. Below, the ratios of pixel densities (NT3:α-tubulin). <b>B:</b> Plots of these ratios confirm this (one symbol per rat) and show a significant decrease after 4 days in NT3 ipsilaterally (I) relative to contralaterally (C) and to normal (Nor). <b>C:</b> Graph of NT3 staining: Each symbol shows the average pixel densities for one rat from 9 regions from capured images (examples in <b>D–H</b>) of a mid-section of an L4 DRG. The plot confirms the findings in <b>B</b>. Nor, C and I defined as for <b>B</b>. It shows a highly significant reduction 4 days after cutaneous CFA-induced inflammation ipsilaterally compared with both contralaterally and with normal. Non-significant decreases in immunoreactivity after 1 day ipsilaterally and contralaterally and after 4 days contralaterally can also be seen. <b>D</b> to <b>H:</b> ABC immunostaining for NT3 in L4 DRGs was most intense in normal rats and least intense ipsilaterally 4 days after CFA-induced inflammation. The staining was in all cell sizes, and diminished in all cell sizes after inflammation.</p>