Effect of temperature on the electrophysiological properties of hippocampal neurons recorded in current-clamp configuration in the presence of synaptic blockers (20 µM CNQX+50 µM AP-V+5 µM bicuculline), which in these abolished cooling-evoked action potential firing. de la PeñaElvira MälkiäAnnika VaraHugo CairesRebeca J. BallestaJuan BelmonteCarlos VianaFelix 2013 <p>The parameters were obtained using depolarizing current pulses of 450-ms-duration at a membrane potential of −60 mV. Membrane resistance was calculated from the I–V slope generated from depolarizing and hyperpolarizing pulses. Statistical significance between parameters obtained at 30°C <i>vs.</i> 35°C was determined with 2-way repeated-measures ANOVA, and indicated with <sup>a</sup> p<0.05; <sup>b</sup> p<0.001; n = 8 for all columns.</p>