Microglial activation. Charleine Zussy Anthony Brureau Emeline Keller Stéphane Marchal Claire Blayo Brice Delair Guy Ixart Tangui Maurice Laurent Givalois 10.1371/journal.pone.0053117.g009 https://plos.figshare.com/articles/figure/_Microglial_activation_/186815 <p><b>A.</b> Effects of oAβ<sub>25–35</sub> (10 µg/rat) icv injection on microglial reaction using Iba-1 immunolabeling in the amygdala, frontal and parietal cortex, hypothalamus (paraventricular nucleus: PVN) and hippocampus (CA1 & CA3 regions) determined in control untreated rats and 6 weeks after Aβ<sub>25–35</sub> scrambled peptide (10 µg/rat; negative control) or Aβ<sub>25–35</sub> injection. Activated microglia was visualized with Alexafluor 488-labeled specific antibody against Iba-1 (green immunolabeling), while the nucleus was counterstained with DAPI (blue labeling). 3v: third ventricle. Scale bar  = 100 µm. <b>B–E.</b> Variations in Iba1 levels in the frontal cortex (B), amygdala (C), hippocampus (D) and hypothalamus (E), determined in rats by western blot 6 weeks after icv injection of scrambled Aβ<sub>25–35</sub> peptide (10 µg/rat; negative control) or oAβ<sub>25–35</sub> (10 µg/rat). Iba1 (17 kDa) variations were normalized with β-tubulin (β-tub, 55 kDa) variations and compared with untreated rats (control group: C). The results are expressed as means ± SEM. *p<0.05 and **p<0.01 <i>vs</i>. control group, +p<0.05 and ++p<0.01 <i>vs</i>. scrambled treated rats. The number of animals in each group is indicated within the columns.</p> 2013-02-19 17:52:13 physiology neuroscience neurological disorders Biochemistry