%0 Figure %A K. Crooke, Adrianne %A R. Fuller, James %A W. Obrist, Markus %A E. Tomkovich, Sarah %A P. Vitko, Nicholas %A R. Richardson, Anthony %D 2013 %T CcpA affect at Pldh1 is indirect. %U https://plos.figshare.com/articles/figure/_CcpA_affect_at_P_ldh1_is_indirect_/179801 %R 10.1371/journal.pone.0054293.g004 %2 https://ndownloader.figshare.com/files/509307 %K genetics and genomics %K microbiology %X

A. EMSA with His-tagged CcpA using Pldh1 (LEFT) or ProcD2 (RIGHT) as probes and an internal hmp fragment as a non-specific probe (N.S. band). Only at highest ratios of CcpA::DNA did non-specific shifted bands become evident using Pldh1 as a probe (white arrows). 250 fmol of DNA probes were used in all wells. B. Alignment of CRE from Pldh1, ProcD2, the B. subtilis consensus sequence and the mutated CRE*. C. Q RT-PCR analyses of ldh1 transcript levels normalized to those of rpoD in WT, ΔccpA and CRE* derivatives of S. aureus strain COL following 15 min. NO· exposure (2 mM DEA-NO).

%I PLOS ONE