Immunohistochemical analysis of Ad5 and Ad5T122 infection in ex vivo tissue cultures of normal human liver and colorectal carcinoma liver metastasis. Erkko Ylösmäki Sergio Lavilla-Alonso Sari Jäämaa Markus Vähä-Koskela Taija af Hällström Akseli Hemminki Johanna Arola Heikki Mäkisalo Kalle Saksela 10.1371/journal.pone.0054506.g003 https://plos.figshare.com/articles/figure/_Immunohistochemical_analysis_of_Ad5_and_Ad5T122_infection_in_ex_vivo_tissue_cultures_of_normal_human_liver_and_colorectal_carcinoma_liver_metastasis_/174066 <p>Precision-cut tumour (top and third row of panels) and liver (second row and bottom panels) tissue cultures were left uninfected (left panels) or infected with 10<sup>7</sup> PFU (in 2 ml of media) of Ad5 (middle panels) or Ad5T122 (right panels), and fixed five days later for staining with haematoxylin-eosin (panels G-L) or an antibody against the viral E1A protein (panels A–F). Necrosis developing in the central area of the uninfected control tissue (panel J) is indicated with arrows.</p> 2013-01-22 01:07:46 ad5 ad5t122 ex vivo cultures colorectal carcinoma