10.1371/journal.pntd.0004302.g002 Renato Sathler-Avelar Renato Sathler-Avelar Danielle Marquete Vitelli-Avelar Danielle Marquete Vitelli-Avelar Armanda Moreira Mattoso-Barbosa Armanda Moreira Mattoso-Barbosa Marcelo Perdigão-de-Oliveira Marcelo Perdigão-de-Oliveira Ronaldo Peres Costa Ronaldo Peres Costa Silvana Maria Elói-Santos Silvana Maria Elói-Santos Matheus de Souza Gomes Matheus de Souza Gomes Laurence Rodrigues do Amaral Laurence Rodrigues do Amaral Andréa Teixeira-Carvalho Andréa Teixeira-Carvalho Olindo Assis Martins-Filho Olindo Assis Martins-Filho Edward J. Dick Jr Edward J. Dick Jr Gene B. Hubbard Gene B. Hubbard Jane F. VandeBerg Jane F. VandeBerg John L. VandeBerg John L. VandeBerg Adaptive immunity features from cynomolgus macaques naturally infected with <i>T</i>. <i>cruzi</i> (CH) and non-infected controls (NI). Public Library of Science 2016 Trypanosoma cruzi Resemble Systems Biology Approaches systems biology analysis expression Human Chagas Disease BackgroundCynomolgus macaques ni nk 15 cynomolgus macaques Chagas disease ch Major Immunological Findings Observed granzyme imbricate biomarker networks 2016-01-28 12:41:27 Figure https://plos.figshare.com/articles/figure/_Adaptive_immunity_features_from_cynomolgus_macaques_naturally_infected_with_T_cruzi_CH_and_non_infected_controls_NI_/1642031 <p>(A) The frequencies of CD3<sup>+</sup> lymphocytes and T-cell subsets (CD4<sup>+</sup> and CD8<sup>+</sup>), the expression of adhesion molecule (CD54) and activation status (CD69 and HLADR) were performed by multicolor flow cytometry. (B) The expression of cytotoxicity markers (Granzyme A, Granzyme B and Perforin) of CD8<sup>+</sup> T-cells was investigated by intracellular staining flow cytometry. (C) Analysis of B-cells, the activation status (CD69), and the expression of the regulatory FcγR (CD32) were evaluated by three-color flow cytometry. The results are expressed as mean percentage with standard error. Significant differences at <i>p<</i>0.05 are identified by (*).</p>