%0 Figure %A Hayez, Aurélie %A Roegiers, Edith %A Malaisse, Jérémy %A Balau, Benoit %A Sterpin, Christiane %A Achouri, Younes %A Lambert De Rouvroit, Catherine %A Poumay, Yves %A Michiels, Carine %A De Backer, Olivier %D 2016 %T Efficient invalidation of TMEM45A in RHE after 11 days of reconstruction (A-C) and in monolayer culture of human keratinocytes (D-F). %U https://plos.figshare.com/articles/figure/_Efficient_invalidation_of_TMEM45A_in_RHE_after_11_days_of_reconstruction_A_C_and_in_monolayer_culture_of_human_keratinocytes_D_F_/1638970 %R 10.1371/journal.pone.0147069.g003 %2 https://ndownloader.figshare.com/files/2629438 %K TMEM 45A knockout mice %K protein %K TMEM 45A %K labelling differentiation markers %K barrier %K TMEM 45A keratinocytes %K epidermal %K TMEM 45A gene expression %K epidermis %K Barrier Formation TMEM 45A gene encodes %X

(A) Relative quantification of TMEM45A mRNA levels in epidermis reconstructed with keratinocytes transduced with NT shRNA or shRNA targeting TMEM45A. Bars represent 95% confidence intervals. Paired t-test (n = 3, **p≤0.1). (B) Morphology of HE-stained epidermis. Bars: 50 μm. (C) Immunofluorescence detection of TMEM45A in RHE. Bars: 50 μm. (D) Relative mRNA quantification in monolayers at subconfluence (SC), confluence (C) and post-confluence (PC). Bars represent 95% confidence intervals. ANOVA 2 (n = 3, *** p≤0.001). (E) TMEM45A abundance analysis by WB in post-confluent monolayer culture. RPL13a is the loading control. (F) Detection of TMEM45A in confluent monolayers culture. Bars: 25 μm. (B, C, E, F). The presented data are representative of the results obtained for at least three independent experiments.

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