10.1371/journal.pone.0147069.g003
Aurélie Hayez
Aurélie
Hayez
Edith Roegiers
Edith
Roegiers
Jérémy Malaisse
Jérémy
Malaisse
Benoit Balau
Benoit
Balau
Christiane Sterpin
Christiane
Sterpin
Younes Achouri
Younes
Achouri
Catherine Lambert De Rouvroit
Catherine
Lambert De Rouvroit
Yves Poumay
Yves
Poumay
Carine Michiels
Carine
Michiels
Olivier De Backer
Olivier
De Backer
Efficient invalidation of <i>TMEM45A</i> in RHE after 11 days of reconstruction (A-C) and in monolayer culture of human keratinocytes (D-F).
Public Library of Science
2016
TMEM 45A knockout mice
protein
TMEM 45A
labelling differentiation markers
barrier
TMEM 45A keratinocytes
epidermal
TMEM 45A gene expression
epidermis
Barrier Formation TMEM 45A gene encodes
2016-01-28 12:37:40
Figure
https://plos.figshare.com/articles/figure/_Efficient_invalidation_of_TMEM45A_in_RHE_after_11_days_of_reconstruction_A_C_and_in_monolayer_culture_of_human_keratinocytes_D_F_/1638970
<p><b>(A)</b> Relative quantification of TMEM45A mRNA levels in epidermis reconstructed with keratinocytes transduced with NT shRNA or shRNA targeting TMEM45A. Bars represent 95% confidence intervals. Paired t-test (n = 3, **p≤0.1). <b>(B)</b> Morphology of HE-stained epidermis. Bars: 50 μm. <b>(C)</b> Immunofluorescence detection of TMEM45A in RHE. Bars: 50 μm. <b>(D)</b> Relative mRNA quantification in monolayers at subconfluence (SC), confluence (C) and post-confluence (PC). Bars represent 95% confidence intervals. ANOVA 2 (n = 3, *** p≤0.001). <b>(E)</b> TMEM45A abundance analysis by WB in post-confluent monolayer culture. RPL13a is the loading control. <b>(F)</b> Detection of TMEM45A in confluent monolayers culture. Bars: 25 μm. (B, C, E, F). The presented data are representative of the results obtained for at least three independent experiments.</p>