10.1371/journal.pone.0147069.g003 Aurélie Hayez Aurélie Hayez Edith Roegiers Edith Roegiers Jérémy Malaisse Jérémy Malaisse Benoit Balau Benoit Balau Christiane Sterpin Christiane Sterpin Younes Achouri Younes Achouri Catherine Lambert De Rouvroit Catherine Lambert De Rouvroit Yves Poumay Yves Poumay Carine Michiels Carine Michiels Olivier De Backer Olivier De Backer Efficient invalidation of <i>TMEM45A</i> in RHE after 11 days of reconstruction (A-C) and in monolayer culture of human keratinocytes (D-F). Public Library of Science 2016 TMEM 45A knockout mice protein TMEM 45A labelling differentiation markers barrier TMEM 45A keratinocytes epidermal TMEM 45A gene expression epidermis Barrier Formation TMEM 45A gene encodes 2016-01-28 12:37:40 Figure https://plos.figshare.com/articles/figure/_Efficient_invalidation_of_TMEM45A_in_RHE_after_11_days_of_reconstruction_A_C_and_in_monolayer_culture_of_human_keratinocytes_D_F_/1638970 <p><b>(A)</b> Relative quantification of TMEM45A mRNA levels in epidermis reconstructed with keratinocytes transduced with NT shRNA or shRNA targeting TMEM45A. Bars represent 95% confidence intervals. Paired t-test (n = 3, **p≤0.1). <b>(B)</b> Morphology of HE-stained epidermis. Bars: 50 μm. <b>(C)</b> Immunofluorescence detection of TMEM45A in RHE. Bars: 50 μm. <b>(D)</b> Relative mRNA quantification in monolayers at subconfluence (SC), confluence (C) and post-confluence (PC). Bars represent 95% confidence intervals. ANOVA 2 (n = 3, *** p≤0.001). <b>(E)</b> TMEM45A abundance analysis by WB in post-confluent monolayer culture. RPL13a is the loading control. <b>(F)</b> Detection of TMEM45A in confluent monolayers culture. Bars: 25 μm. (B, C, E, F). The presented data are representative of the results obtained for at least three independent experiments.</p>