10.1371/journal.pgen.1005779.g002 Ilaria Dalla Rosa Ilaria Dalla Rosa Yolanda Cámara Yolanda Cámara Romina Durigon Romina Durigon Chloe F. Moss Chloe F. Moss Sara Vidoni Sara Vidoni Gokhan Akman Gokhan Akman Lilian Hunt Lilian Hunt Mark A. Johnson Mark A. Johnson Sarah Grocott Sarah Grocott Liya Wang Liya Wang David R. Thorburn David R. Thorburn Michio Hirano Michio Hirano Joanna Poulton Joanna Poulton Robert W. Taylor Robert W. Taylor Greg Elgar Greg Elgar Ramon Martí Ramon Martí Peter Voshol Peter Voshol Ian J. Holt Ian J. Holt Antonella Spinazzola Antonella Spinazzola Mpv17<sup>-/-</sup> mouse liver mitochondria have significantly reduced levels of two precursors of DNA synthesis, dGTP and dTTP. Public Library of Science 2016 dNTP levels Mitochondria MPV 17 mitochondrial DNA loss MPV 17 deficiency mitochondrial DNA depletion dysfunction causes mitochondrial DNA abnormalities MPV 17 Loss Causes Deoxynucleotide Insufficiency deoxynucleoside supplementation Slow DNA Replication mitochondrial deoxynucleotide homeostasis mitochondrial genomic instability DNA copy number MPV 17 disease models 2016-01-18 15:33:16 Figure https://plos.figshare.com/articles/figure/_Mpv17_mouse_liver_mitochondria_have_significantly_reduced_levels_of_two_precursors_of_DNA_synthesis_dGTP_and_dTTP_/1637040 <p>The abundance of dNTPs was determined by means of an extension assay after extraction of nucleotides from liver (<b>A</b>), kidney (<b>B</b>) or brain (<b>C</b>) of <i>Mpv17</i><sup><i>+/+</i></sup> and <i>Mpv17</i><sup><i>-/-</i></sup> mice (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005779#sec010" target="_blank">Methods</a>). All mice (n = 5) were sacrificed between 8 and 10 weeks of age. P values were obtained using Mann-Whitney test (* P<0.05, NS, not significant P>0.05).</p>