%0 Figure %A Abou Hammoud, Aya %A Kirstein, Nina %A Mournetas, Virginie %A Darracq, Anais %A Broc, Sabine %A Blanchard, Camille %A Zeineddine, Dana %A Mortada, Mohamad %A Boeuf, Helene %D 2016 %T Klf5 but not JunB disturbs the LIF-dependent plasticity in early committed cells. %U https://plos.figshare.com/articles/figure/_Klf5_but_not_JunB_disturbs_the_LIF_dependent_plasticity_in_early_committed_cells_/1633233 %R 10.1371/journal.pone.0146281.g003 %2 https://ndownloader.figshare.com/files/2621234 %K pluripotency %K Master gene expression %K 3k %K plasticity %K pi %K Metalloproteinase MMP 1 %K LIF withdrawal %K mesodermal marker Brachyury %K displays pleiotropic functions %K mesc %K IL 6 cytokine family member %K neuroectoderm lineage markers %K Murine Embryonic Stem Cell Plasticity %K MMP 1 metalloproteinase %X

Cells were depleted from LIF for 24 hours and either transfected with siControl (siCtrl) (A) siKlf5, or (B) siJunB and then induced with LIF for three days (d3). Graphs represent the average level of expression and SEM from 3 to 6 independent experiments. Paired sample t-test was performed to determine the significance of the difference in the expression levels observed with the non specific (siCtrl) versus specific siRNA: *p-value<0.05; **p-value<0.01, ***p-value<0.001; if not stated: not significant.

%I PLOS ONE