10.1371/journal.pone.0146281.g002 Aya Abou Hammoud Aya Abou Hammoud Nina Kirstein Nina Kirstein Virginie Mournetas Virginie Mournetas Anais Darracq Anais Darracq Sabine Broc Sabine Broc Camille Blanchard Camille Blanchard Dana Zeineddine Dana Zeineddine Mohamad Mortada Mohamad Mortada Helene Boeuf Helene Boeuf LIF has differential effects on mESC-derived cells, after d1, d2 or d3 of LIF starvation. Public Library of Science 2016 pluripotency Master gene expression 3k plasticity pi Metalloproteinase MMP 1 LIF withdrawal mesodermal marker Brachyury displays pleiotropic functions mesc IL 6 cytokine family member neuroectoderm lineage markers Murine Embryonic Stem Cell Plasticity MMP 1 metalloproteinase 2016-01-18 14:26:07 Figure https://plos.figshare.com/articles/figure/_LIF_has_differential_effects_on_mESC_derived_cells_after_d1_d2_or_d3_of_LIF_starvation_/1633232 <p>Cells were depleted from LIF or induced with LIF after a period of LIF depletion as indicated. After 4 days, expression of the selected genes (A) <i>Master</i> genes, (B) <i>Pluri</i> genes and (C) <i>Diff</i> genes was analyzed by RT-qPCR with <i>Hprt</i> used for normalization. Graphs represent the average level of expression and standard error of the mean (SEM) bars, calculated from 4 independent experiments. +LIF condition was arbitrarily set as 1. One sample t-test was performed for each condition versus the +LIF sample: *p-value<0.05; **p-value<0.01, ***p-value<0.001; a: p-value = 0.051; if not stated: not significant. (D) A representative experiment of flow cytometry, performed in the indicated conditions with the Ceacam-PE antibody, is presented: "off set graph" of the MFI of the total P1 gated cell population of each cell growth condition, with the Flo Jo software.</p>