10.1371/journal.pone.0146281.g002
Aya Abou Hammoud
Aya
Abou Hammoud
Nina Kirstein
Nina
Kirstein
Virginie Mournetas
Virginie
Mournetas
Anais Darracq
Anais
Darracq
Sabine Broc
Sabine
Broc
Camille Blanchard
Camille
Blanchard
Dana Zeineddine
Dana
Zeineddine
Mohamad Mortada
Mohamad
Mortada
Helene Boeuf
Helene
Boeuf
LIF has differential effects on mESC-derived cells, after d1, d2 or d3 of LIF starvation.
Public Library of Science
2016
pluripotency
Master gene expression
3k
plasticity
pi
Metalloproteinase MMP 1
LIF withdrawal
mesodermal marker Brachyury
displays pleiotropic functions
mesc
IL 6 cytokine family member
neuroectoderm lineage markers
Murine Embryonic Stem Cell Plasticity
MMP 1 metalloproteinase
2016-01-18 14:26:07
Figure
https://plos.figshare.com/articles/figure/_LIF_has_differential_effects_on_mESC_derived_cells_after_d1_d2_or_d3_of_LIF_starvation_/1633232
<p>Cells were depleted from LIF or induced with LIF after a period of LIF depletion as indicated. After 4 days, expression of the selected genes (A) <i>Master</i> genes, (B) <i>Pluri</i> genes and (C) <i>Diff</i> genes was analyzed by RT-qPCR with <i>Hprt</i> used for normalization. Graphs represent the average level of expression and standard error of the mean (SEM) bars, calculated from 4 independent experiments. +LIF condition was arbitrarily set as 1. One sample t-test was performed for each condition versus the +LIF sample: *p-value<0.05; **p-value<0.01, ***p-value<0.001; a: p-value = 0.051; if not stated: not significant. (D) A representative experiment of flow cytometry, performed in the indicated conditions with the Ceacam-PE antibody, is presented: "off set graph" of the MFI of the total P1 gated cell population of each cell growth condition, with the Flo Jo software.</p>