Formation of second-generation free-living larvae in <i>Strongyloides stercoralis</i> requires 24–48 hours of exposure to Δ7-dafachronic acid.
Mennatallah M. Y. Albarqi
Jonathan D. Stoltzfus
Adeiye A. Pilgrim
Thomas J. Nolan
Zhu Wang
Steven A. Kliewer
David J. Mangelsdorf
James B. Lok
10.1371/journal.ppat.1005358.g004
https://plos.figshare.com/articles/figure/_Formation_of_second_generation_free_living_larvae_in_Strongyloides_stercoralis_requires_24_48_hours_of_exposure_to_7_dafachronic_acid_/1632488
<p>Frequency of development to second-generation free-living rhabditiform L3 and L4 (FL L3-L4) in <i>S</i>. <i>stercoralis</i> was plotted as a function of duration of exposure to increasing concentrations of Δ7-dafachronic acid (Δ7-DA). Ascending shaded triangles indicate that developmental frequencies were determined at Δ7-DA concentrations of 125 nM (lightest), 250 nM, 500 nM, and 1,000 nM (darkest). Bar height represents the mean of three biological replicates; error bars represent +1 standard deviation. The overall effects of Δ7-DA exposure duration and concentration were significant, P < 0.0001. For all possible pairwise statistical comparisons of FL L3-L4 frequency with DA exposure durations of 24, 48, and 72 hours, ** P < 0.01; *** P < 0.001.</p>
2016-01-05 14:56:13
CYP inhibitor ketoconazole
1 μ M Δ7- DA
post-parasitic first-stage larvae
post-parasitic L 1s
iL 3 activation
400 nM Δ7- DA
nematode Strongyloides stercoralis
hormone receptor DAF -12. Biosynthesis
DAF -9.
35 μ M ketoconazole
26 CYP-encoding genes
free-living nematode Caenorhabditis elegans
DAF -12
iL 3 arrest
DA production
SD
Δ7- DA exposure
40 μ M ketoconazole
stercoralis development
iL 3
cytochrome P 450
post-free-living iL 3 arrest
Life Cycle Checkpoints