10.1371/journal.pgen.1005759.g002
Sabine S. Lange
Sabine
S. Lange
Junya Tomida
Junya
Tomida
Karen S. Boulware
Karen
S. Boulware
Sarita Bhetawal
Sarita
Bhetawal
Richard D. Wood
Richard D.
Wood
Spontaneous DNA double-strand break formation is reduced in <i>Rev3l</i> complemented MEFs.
Public Library of Science
2016
chicken DT 40 cells
mouse fibroblast cell lines
REV 3L DNA polymerase activity
Rev 3l gene encoding
Rev 3l cDNA rescues
DNA polymerase activity
DNA damage sensitivity
Embryonic Viability DNA polymerase ζ
REV 3L protein
translesion DNA synthesis
Rev 3l mice
Rev 3l phenotypes
REV 3L
Rev 3l fibroblasts
2016-01-05 14:56:07
Figure
https://plos.figshare.com/articles/figure/_Spontaneous_DNA_double_strand_break_formation_is_reduced_in_Rev3l_complemented_MEFs_/1632391
<p>(A) DAPI staining of empty vector (EV)-expressing <i>Rev3l</i><sup>+/Δ</sup>, <i>Rev3l</i><sup>-/Δ</sup>, as well as <i>Rev3l</i><sup>-/Δ</sup> MEFs expressing wild-type or ASM recombinant <i>REV3L</i>; arrows indicate micronuclei, with an enlarged example in the inset. (B) Merged immunofluorescence staining of the same MEFs as in (A) with DAPI (blue), 53BP1 (red) and γ-H2AX (green); foci indicate areas of DNA double-strand breaks. (C) Quantification of percent of nuclei that have associated micronuclei. (D) Quantification of cells with fewer than 3, 3 to 5, 6 to 10 or greater than 10 53BP1 foci (as measured using CellProfiler). The bars are color-coded exactly as in Part C to indicate the genotype of the MEFs (*) p < 0.01. Data represent mean ± SEM.</p>