10.1371/journal.pgen.1005759.g002 Sabine S. Lange Sabine S. Lange Junya Tomida Junya Tomida Karen S. Boulware Karen S. Boulware Sarita Bhetawal Sarita Bhetawal Richard D. Wood Richard D. Wood Spontaneous DNA double-strand break formation is reduced in <i>Rev3l</i> complemented MEFs. Public Library of Science 2016 chicken DT 40 cells mouse fibroblast cell lines REV 3L DNA polymerase activity Rev 3l gene encoding Rev 3l cDNA rescues DNA polymerase activity DNA damage sensitivity Embryonic Viability DNA polymerase ζ REV 3L protein translesion DNA synthesis Rev 3l mice Rev 3l phenotypes REV 3L Rev 3l fibroblasts 2016-01-05 14:56:07 Figure https://plos.figshare.com/articles/figure/_Spontaneous_DNA_double_strand_break_formation_is_reduced_in_Rev3l_complemented_MEFs_/1632391 <p>(A) DAPI staining of empty vector (EV)-expressing <i>Rev3l</i><sup>+/Δ</sup>, <i>Rev3l</i><sup>-/Δ</sup>, as well as <i>Rev3l</i><sup>-/Δ</sup> MEFs expressing wild-type or ASM recombinant <i>REV3L</i>; arrows indicate micronuclei, with an enlarged example in the inset. (B) Merged immunofluorescence staining of the same MEFs as in (A) with DAPI (blue), 53BP1 (red) and γ-H2AX (green); foci indicate areas of DNA double-strand breaks. (C) Quantification of percent of nuclei that have associated micronuclei. (D) Quantification of cells with fewer than 3, 3 to 5, 6 to 10 or greater than 10 53BP1 foci (as measured using CellProfiler). The bars are color-coded exactly as in Part C to indicate the genotype of the MEFs (*) p < 0.01. Data represent mean ± SEM.</p>