10.1371/journal.pgen.1003275.g007
Martin Christmann
Martin
Christmann
Tilo Schmaler
Tilo
Schmaler
Colin Gordon
Colin
Gordon
Xiaohua Huang
Xiaohua
Huang
Özgür Bayram
Özgür
Bayram
Josua Schinke
Josua
Schinke
Sina Stumpf
Sina
Stumpf
Wolfgang Dubiel
Wolfgang
Dubiel
Gerhard H. Braus
Gerhard H.
Braus
CSN targets DEN1/DenA for degradation in fungal and human cells.
Public Library of Science
2013
targets
degradation
fungal
2013-02-07 00:47:22
Figure
https://plos.figshare.com/articles/figure/_CSN_targets_DEN1_DenA_for_degradation_in_fungal_and_human_cells_/162842
<p>(A) Quantitative analysis of repeated western blot experiments displayed the differences in DenA abundance in fungal wild type and <i>ΔcsnG</i> cells. DenA levels of the different asexual developmental time points are shown relative to the vegetative (veg.) control for each strain. Anti-Actin was applied as loading control (statistics: 2-way ANOVA; n = 3; *p<0,05, **p<0,01). (B) Xpress-DEN1 was overexpressed in siGFP and siCSN1 human cells and steady state Xpress-DEN1 levels were estimated by western analysis with the α-Xpress antibody. Xpress-CSN1 was overexpressed in siCSN1 cells and DEN1 and CSN8 were probed with appropriate antibodies. (C) Xpress-DEN1 was overexpressed in siGFP human cells and the proteasome inhibitor MG132 was added 6 h before cell lysis at a final concentration of 10 µM. Cyclohexamide (CHX) was added in a final concentration of 10 µg/ml (D) Xpress-DEN1 was co-expressed in HeLa cells together with Xpress-CSN1wt, Xpress-CSN1(1–221) or Xpress-CSN1(222–527) in the absence or in the presence of MG132 (right panel), which was added 6 h before cell lysis. Cells were lyzed 24 h after co-transfection and lysates were analyzed by western blot using the α-DEN1 antibody (0 = only Xpress-DEN1).</p>