10.1371/journal.pone.0145259.g003
Greer K. Arthur
Greer
K. Arthur
S. Mark Duffy
S.
Mark Duffy
Katy M. Roach
Katy
M. Roach
Rob A. Hirst
Rob
A. Hirst
Aarti Shikotra
Aarti
Shikotra
Erol A. Gaillard
Erol
A. Gaillard
Peter Bradding
Peter
Bradding
Freshly isolated HBECs, and the H292 and BEAS-2B airway epithelial cell lines exhibit K<sub>Ca</sub>3.1 channel activity.
Public Library of Science
2015
KCa 3.1 blockade
KCa 3.1 ion currents
KCa 3.1 blockers
Primary HBECs
epithelial cell proliferation
tgf
epithelial fluid secretion
KCa 3.1 mRNA
HBEC function
KCa 3.1 currents
emt
H 292 cells
airway epithelial biology
Selective KCa 3.1 blockers
tram
Human Bronchial Epithelial Cells
neoplastic H 292 epithelial cell line
KCa 3.1 protein expression
ica
KCa 3.1 channel
beas
KCa 3.1 K
2015-12-23 10:06:18
Figure
https://plos.figshare.com/articles/figure/_Freshly_isolated_HBECs_and_the_H292_and_BEAS_2B_airway_epithelial_cell_lines_exhibit_K_Ca_3_1_channel_activity_/1627513
<p>Current-voltage curves demonstrating that 100 μM 1-EBIO induced characteristic K<sub>Ca</sub>3.1 channel currents in freshly isolated HBECs isolated from (<b>A</b>) one patient with asthma (n = 3 cells) and (<b>B</b>) one healthy control (n = 3 cells). TRAM-34 (200 nM) blocked 1-EBIO-induced currents to near-baseline levels. Characteristic 1-EBIO-dependent K<sub>Ca</sub>3.1 channel activity, sensitive to TRAM-34, was also seen in (<b>C</b>) the H292 cell line (n = 6 cells) and (<b>D</b>) the BEAS-2B cell line (n = 8 cells).</p>