10.1371/journal.pone.0145259.g003 Greer K. Arthur Greer K. Arthur S. Mark Duffy S. Mark Duffy Katy M. Roach Katy M. Roach Rob A. Hirst Rob A. Hirst Aarti Shikotra Aarti Shikotra Erol A. Gaillard Erol A. Gaillard Peter Bradding Peter Bradding Freshly isolated HBECs, and the H292 and BEAS-2B airway epithelial cell lines exhibit K<sub>Ca</sub>3.1 channel activity. Public Library of Science 2015 KCa 3.1 blockade KCa 3.1 ion currents KCa 3.1 blockers Primary HBECs epithelial cell proliferation tgf epithelial fluid secretion KCa 3.1 mRNA HBEC function KCa 3.1 currents emt H 292 cells airway epithelial biology Selective KCa 3.1 blockers tram Human Bronchial Epithelial Cells neoplastic H 292 epithelial cell line KCa 3.1 protein expression ica KCa 3.1 channel beas KCa 3.1 K 2015-12-23 10:06:18 Figure https://plos.figshare.com/articles/figure/_Freshly_isolated_HBECs_and_the_H292_and_BEAS_2B_airway_epithelial_cell_lines_exhibit_K_Ca_3_1_channel_activity_/1627513 <p>Current-voltage curves demonstrating that 100 μM 1-EBIO induced characteristic K<sub>Ca</sub>3.1 channel currents in freshly isolated HBECs isolated from (<b>A</b>) one patient with asthma (n = 3 cells) and (<b>B</b>) one healthy control (n = 3 cells). TRAM-34 (200 nM) blocked 1-EBIO-induced currents to near-baseline levels. Characteristic 1-EBIO-dependent K<sub>Ca</sub>3.1 channel activity, sensitive to TRAM-34, was also seen in (<b>C</b>) the H292 cell line (n = 6 cells) and (<b>D</b>) the BEAS-2B cell line (n = 8 cells).</p>