10.1371/journal.pntd.0004189.g008
Silvia B. Bazan
Silvia
B. Bazan
Tania A. Costa
Tania A.
Costa
Eliseu Frank de Araújo
Eliseu
Frank de Araújo
Claudia Feriotti
Claudia
Feriotti
Flávio V. Loures
Flávio
V. Loures
Fernando D. Pretel
Fernando
D. Pretel
Vera L. G. Calich
Vera
L. G. Calich
Treg cells limit inflammation and increase the differentiation of Th1, Th2 and Th17 cells.
Public Library of Science
2015
3GFP
impairing T cell immunity
Treg cells
T Cell Immunity
tissue inflammation
pcm
regulatory t cells
106 yeast cells
Dual Role Exerted
Pulmonary Paracoccidioidomycosis Paracoccidioidomycosis
limit pathogen growth
T cell subsets
2015-10-29 03:23:37
Figure
https://plos.figshare.com/articles/figure/_Treg_cells_limit_inflammation_and_increase_the_differentiation_of_Th1_Th2_and_Th17_cells_/1589257
<p>Rag1<sup>-/-</sup> mice were reconstituted with different T cell subpopulations, infected with <i>P</i>. <i>brasiliensis</i> and sacrificed at the time points indicated. Lungs were excised and single cells were stained as described in Materials and Methods section. (A) Number of total leukocytes, lymphocytes, macrophages and granulocytes in the lungs of infected Rag1<sup>-/-</sup> mice given Treg cells, CD4<sup>+</sup>Foxp3<sup>-</sup> T cells or CD4<sup>+</sup>Foxp3<sup>-</sup> T cells + Treg cells. (B) Frequency and number of CD4<sup>+</sup>CD69<sup>+</sup>Foxp3<sup>-</sup> T lymphocytes and Treg cells in the lungs of infected Rag1<sup>-/-</sup> mice previously reconstituted with Treg cells, CD4<sup>+</sup>Foxp3<sup>-</sup> T cells or CD4<sup>+</sup>Foxp3<sup>-</sup> T cells + Treg cells. (C-E) Numbers of CD4<sup>+</sup> T lymphocytes producing IFN-γ, IL-17 and IL-4 in reconstituted and infected Rag1<sup>-/-</sup> mice. (F) Quantitative PCR analysis of major transcription factors of T cells in the lungs of Rag1<sup>-/-</sup> mice, as determined by RT-PCR after 2 and 6 weeks post-infection. Rag1<sup>-/-</sup> mice were reconstituted with the different T cell subpopulations or vehicle only, infected with <i>P</i>. <i>brasiliensis</i> or left uninfected, and sacrificed after 2 and 6 weeks. Lungs were removed, RNA was extracted, reverse transcribed and the amplified cDNA was used for RT-PCR, as described previously. All bars indicate mean ± SD from at least 5 mice per group and are representative of three independent experiments (* <i>p</i> < 0.05; **<i>p</i> < 0.005; ***<i>p</i> < 0.001).</p>