10.1371/journal.pone.0141696.g008 Sophie Jamet Sophie Jamet Jaclyn Bubnell Jaclyn Bubnell Patrick Pfister Patrick Pfister Delia Tomoiaga Delia Tomoiaga Matthew E. Rogers Matthew E. Rogers Paul Feinstein Paul Feinstein Plot for relative EC50 and % of maximum response. Public Library of Science 2015 plasma membrane localization m β2AR mutants m β2AR axon guidance studies or transmembrane domain 7 plasma membrane trafficking 327R plasma membrane GPCR motif NPxxY rdy 268A ct Vivo Surrogate Odorant Receptor Vitro Mutational Analysis m β2AR EC 50s β2 Adrenergic Receptor m β2AR agonist 2015-10-29 03:17:44 Figure https://plos.figshare.com/articles/figure/_Plot_for_relative_EC50_and_of_maximum_response_/1589245 <p>All mutants are positioned for their relative EC50 for isoprenaline (EC50/EC50 of WT) on the y-axis (log scale) and their maximum response as a % of WT on the x-axis. Mutants that fail to traffic to the plasma membrane are depicted as red diamonds and mutants that properly traffic to the plasma membrane are depicted as green diamonds. The red box indicates all the mutants with a lower affinity for isoprenaline than WT i.e. EC50/EC50 of WT >>1 (from 3.9 to 77.4). All these mutants have mutations in the last 7<sup>th</sup> transmembrane domain or the Ct of the protein. The black box includes all the mutants that have a similar affinity for isoprenaline than WT i.e. EC50/EC50 of β2AR::GFP ≈ 1 (from 0.8 to 2.1).</p>