10.1371/journal.pone.0141696.g008
Sophie Jamet
Sophie
Jamet
Jaclyn Bubnell
Jaclyn
Bubnell
Patrick Pfister
Patrick
Pfister
Delia Tomoiaga
Delia
Tomoiaga
Matthew E. Rogers
Matthew E.
Rogers
Paul Feinstein
Paul
Feinstein
Plot for relative EC50 and % of maximum response.
Public Library of Science
2015
plasma membrane localization
m β2AR mutants
m β2AR
axon guidance studies
or
transmembrane domain 7
plasma membrane trafficking
327R
plasma membrane
GPCR motif NPxxY
rdy
268A
ct
Vivo Surrogate Odorant Receptor
Vitro Mutational Analysis
m β2AR EC 50s
β2 Adrenergic Receptor
m β2AR agonist
2015-10-29 03:17:44
Figure
https://plos.figshare.com/articles/figure/_Plot_for_relative_EC50_and_of_maximum_response_/1589245
<p>All mutants are positioned for their relative EC50 for isoprenaline (EC50/EC50 of WT) on the y-axis (log scale) and their maximum response as a % of WT on the x-axis. Mutants that fail to traffic to the plasma membrane are depicted as red diamonds and mutants that properly traffic to the plasma membrane are depicted as green diamonds. The red box indicates all the mutants with a lower affinity for isoprenaline than WT i.e. EC50/EC50 of WT >>1 (from 3.9 to 77.4). All these mutants have mutations in the last 7<sup>th</sup> transmembrane domain or the Ct of the protein. The black box includes all the mutants that have a similar affinity for isoprenaline than WT i.e. EC50/EC50 of β2AR::GFP ≈ 1 (from 0.8 to 2.1).</p>