R. V. Hammond, Gerald Takasuga, Shunsuke Sasaki, Takehiko Balla, Tamas The GFP-ML1Nx2 probe labels punctate structures even in the absence of PIKfyve. <p>Images show a representative wild-type and PIKfyve knock-out (KO) ES cells expressing the GFP-ML1Nx2 probe. Note the fluorescent puncta in the PIKfyve KO cell which showed swollen endosomes, a hallmark of PtdIns(3,5)<i>P</i><sub>2</sub> deficiency. Data are representative of 11 cells, scale bar = 15 μm.</p> ML 1Nx;Eukaryotic cells;probe;phosphatidylinositol;endocytic membrane traffic;organism physiology;biosensor;localization;PtdIn;genomic interventions 2015-10-13
    https://plos.figshare.com/articles/figure/_The_GFP_ML1Nx2_probe_labels_punctate_structures_even_in_the_absence_of_PIKfyve_/1573632
10.1371/journal.pone.0139957.g005