TY - DATA T1 - Premature termination of GAT1 transcription explains paradoxical negative correlation between nitrogen-responsive mRNA, but constitutive low-level protein production PY - 2015/10/16 AU - Georis Isabelle AU - Jennifer J Tate AU - Fabienne Vierendeels AU - Terrance G Cooper AU - Evelyne Dubois UR - https://tandf.figshare.com/articles/dataset/Premature_termination_of_i_GAT1_i_transcription_explains_paradoxical_negative_correlation_between_nitrogen_responsive_mRNA_but_constitutive_low_level_protein_production/1569221 DO - 10.6084/m9.figshare.1569221.v4 L4 - https://ndownloader.figshare.com/files/2351630 L4 - https://ndownloader.figshare.com/files/2351631 L4 - https://ndownloader.figshare.com/files/2351632 L4 - https://ndownloader.figshare.com/files/2351633 L4 - https://ndownloader.figshare.com/files/2351634 L4 - https://ndownloader.figshare.com/files/2351635 L4 - https://ndownloader.figshare.com/files/2351636 L4 - https://ndownloader.figshare.com/files/2351637 KW - mRNA termini KW - level production KW - protein production KW - GAT 1 gene KW - growth conditions KW - alternative transcription initiation KW - transcriptional termination KW - nitrogen catabolite repression KW - Premature termination KW - length proteins KW - GAT 1 transcription KW - transcriptional activator KW - constitutive KW - ncr N2 - The first step in executing the genetic program of a cell is production of mRNA. In yeast, almost every gene is transcribed as multiple distinct isoforms, differing at their 5′ and/or 3′ termini. However, the implications and functional significance of the transcriptome-wide diversity of mRNA termini remains largely unexplored. In this paper, we show that the GAT1 gene, encoding a transcriptional activator of nitrogen-responsive catabolic genes, produces a variety of mRNAs differing in their 5′ and 3′ termini. Alternative transcription initiation leads to the constitutive, low level production of 2 full length proteins differing in their N-termini, whereas premature transcriptional termination generates a short, highly nitrogen catabolite repression- (NCR-) sensitive transcript that, as far as we can determine, is not translated under the growth conditions we used, but rather likely protects the cell from excess Gat1. ER -