10.6084/m9.figshare.1569197.v1 Hwee Ying Lim Hwee Ying Lim Qin Shi Ho Qin Shi Ho Kim Ping Wong Kim Ping Wong Interplay of metabolizing enzymes and transporter of xenobiotics Taylor & Francis Group 2015 sulfate glutathione paps bcrp breast cancer resistance protein atp efflux biosynthesi conjugate abc xenobiotic transporter Phase II detoxification metabolizing enzymes 2015-07-30 00:00:00 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Interplay_of_metabolizing_enzymes_and_transporter_of_xenobiotics/1569197 <div><p></p><p>1. Xenobiotics are metabolized and eliminated through the coordinated interplay of their metabolizing enzymes and transporters. However, these two activities <i>in vitro</i> are measured separately, with the addition of ATP as a pre-requisite.</p><p>2. We propose a human renal cell-line model which integrates the sulfate and glutathione conjugation of xenobiotics with the efflux of their respective conjugates. Sulfation and glutathionylation represent two major Phase II detoxification of xenobiotics in man. The reactions are catalyzed, respectively, by phenolsulfotransferase and glutathione-<i>S</i>-transferase followed by extrusion of their respective conjugates.</p><p>3. Using Ko-143, a specific inhibitor of breast cancer resistance protein (BCRP), an ATP-binding cassette (ABC) transporter, we identified this transporter to be responsible for the efflux of <i>p</i>-cresol sulfate, harmol sulfate and the glutathione conjugate of 1-chloro-2,4-dinitrobenzene.</p><p>4. The conjugation-cum-efflux was inhibited by oligomycin and uncouplers, which highlights the role of cellular mitochondria in providing ATP for the biosynthesis of their conjugating agents, 3′-phosphoadenosine-5′-phosphosulfate (PAPS) and reduced glutathione as well as for the transport function of BCRP.</p><p>5. The human 786-O renal cell-line provides a “3-in-1” system linking ATP biosynthesis to metabolism of xenobiotics and their ultimate transport and elimination by BCRP; this integrated system was not apparent in other human cell-lines examined.</p></div>