Glover, Kyle P. Chen, Zhongqiang K. Markell, Lauren Han, Xing Validation of seventeen gene signature with other PKC-activating tumor promoters. <p>TK6 cells were treated with other PKC-activating tumor promoters including PDBu, SPd, Mez, IndV and ROPA and exposed to UVC-irradiation to verify the 17 gene signature observed with RNA-sequencing. Gene expression was observed with QPCR. Most of the 17 genes had synergistic expression patterns in the co-treated cells (green bar). The fold-change of the UVC-alone (grey bar) and PKC-activator alone (red bar) are stacked to represent the theoretical additive response. One way ANOVA with Bonferroni post-hoc analysis was conducted (p<0.05) to determine statistical significance of the co-treated cells compared to either stress alone.</p> TK 6 cells;ROPA;tpa;Human lymphoblastoid TK 6 cells;uvc;pcr;protein kinase C;17 gene signature;ap;gene expression patterns;Synergistic Gene Expression Signature Observed;dna;stress response pathways;novel gene signature;tumor microenvironment;qpcr;gene expression signatures 2015-10-02
    https://plos.figshare.com/articles/figure/_Validation_of_seventeen_gene_signature_with_other_PKC_activating_tumor_promoters_/1562988
10.1371/journal.pone.0139850.g009