Genome-Wide Association Study for Autism Spectrum Disorder in Taiwanese Han Population Po-Hsiu Kuo Li-Chung Chuang Mei-Hsin Su Chia-Hsiang Chen Chien-Hsiun Chen Jer-Yuarn Wu Chung-Jen Yen Yu-Yu Wu Shih-Kai Liu Miao-Chun Chou Wen-Jiun Chou Yen-Nan Chiu Wen-Che Tsai Susan Shur-Fen Gau 10.1371/journal.pone.0138695 https://plos.figshare.com/articles/dataset/_Genome_Wide_Association_Study_for_Autism_Spectrum_Disorder_in_Taiwanese_Han_Population_/1554103 <div><p>Background</p><p>Autism spectrum disorder (ASD) is a neurodevelopmental disorder with strong genetic components. Several recent genome-wide association (GWA) studies in Caucasian samples have reported a number of gene regions and loci correlated with the risk of ASD—albeit with very little consensus across studies.</p><p>Methods</p><p>A two-stage GWA study was employed to identify common genetic variants for ASD in the Taiwanese Han population. The discovery stage included 315 patients with ASD and 1,115 healthy controls, using the Affymetrix SNP array 6.0 platform for genotyping. Several gene regions were then selected for fine-mapping and top markers were examined in extended samples. Single marker, haplotype, gene-based, and pathway analyses were conducted for associations.</p><p>Results</p><p>Seven SNPs had p-values ranging from 3.4~9.9*10<sup>−6</sup>, but none reached the genome-wide significant level. Five of them were mapped to three known genes (<i>OR2M4</i>, <i>STYK1</i>, and <i>MNT</i>) with significant empirical gene-based p-values in <i>OR2M4</i> (p = 3.4*10<sup>−5</sup>) and <i>MNT</i> (p = 0.0008). Results of the fine-mapping study showed single-marker associations in the <i>GLIS1</i> (rs12082358 and rs12080993) and <i>NAALADL2</i> (rs3914502 and rs2222447) genes, and gene-based associations for the <i>OR2M3-OR2T5</i> (olfactory receptor genes, p = 0.02), and <i>GLIPR1/KRR1</i> gene regions (p = 0.015). Pathway analyses revealed important pathways for ASD, such as olfactory and G protein–coupled receptors signaling pathways.</p><p>Conclusions</p><p>We reported Taiwanese Han specific susceptibility genes and variants for ASD. However, further replication in other Asian populations is warranted to validate our findings. Investigation in the biological functions of our reported genetic variants might also allow for better understanding on the underlying pathogenesis of autism.</p></div> 2015-09-23 03:41:58 Taiwanese Han population Affymetrix SNP array 6.0 platform Autism Spectrum Disorder 2m neurodevelopmental disorder discovery stage mnt rs 12082358 gene regions gwa associations.ResultsSeven SNPs susceptibility genes asd Caucasian samples Taiwanese Han STYK rs 3914502 receptor genes Asian populations Taiwanese Han Population BackgroundAutism spectrum disorder GLIS 1 Several gene regions variant 315 patients pathway analyses Single marker NAALADL 2 GLIPR