Ai, Minrong Mills, Holly Kanai, Makoto Lai, Jason Deng, Jingjing Schreiter, Eric Looger, Loren Neubert, Thomas Suh, Greg GCaMP3 is converted from green to red fluorescence after exposure to blue light. <p><b>(A-B)</b> Fluorescent micrographs of dissected <i>Drosophila</i> brains expressing UAS-GCaMP3; <i>IR8a</i>-GAL4 <b>(A)</b> or UAS-GCaMP6s; NP225-GAL4 <b>(B)</b>. The dissected brains were exposed to blue light (mercury arc light passed through a Zeiss 63x oil-immersion objective) for different durations as indicated at the top of each panel. Green (top) and red (bottom) fluorescence micrographs were captured using a confocal microscope. Scale bar: 20μm. <b>(C)</b> Green-to-red photoconversion of GCaMP3 as quantified by the ratio of red-to-green fluorescence intensity (y-axis) following exposure to light from a Xenon lamp (x-axis).</p> GCaMP variant;acid residues;imaging intracellular calcium transients;GECI;cell culture;GCaMP Genetically;GCaMP reagents;oxygen environment;photoconversion efficiency;intracellular calcium concentrations;calcium responsiveness;calcium indicators 2015-09-18
    https://plos.figshare.com/articles/figure/_GCaMP3_is_converted_from_green_to_red_fluorescence_after_exposure_to_blue_light_/1549243
10.1371/journal.pone.0138127.g001