GCaMP3 is converted from green to red fluorescence after exposure to blue light. Minrong Ai Holly Mills Makoto Kanai Jason Lai Jingjing Deng Eric Schreiter Loren Looger Thomas Neubert Greg Suh 10.1371/journal.pone.0138127.g001 https://plos.figshare.com/articles/figure/_GCaMP3_is_converted_from_green_to_red_fluorescence_after_exposure_to_blue_light_/1549243 <p><b>(A-B)</b> Fluorescent micrographs of dissected <i>Drosophila</i> brains expressing UAS-GCaMP3; <i>IR8a</i>-GAL4 <b>(A)</b> or UAS-GCaMP6s; NP225-GAL4 <b>(B)</b>. The dissected brains were exposed to blue light (mercury arc light passed through a Zeiss 63x oil-immersion objective) for different durations as indicated at the top of each panel. Green (top) and red (bottom) fluorescence micrographs were captured using a confocal microscope. Scale bar: 20μm. <b>(C)</b> Green-to-red photoconversion of GCaMP3 as quantified by the ratio of red-to-green fluorescence intensity (y-axis) following exposure to light from a Xenon lamp (x-axis).</p> 2015-09-18 03:49:16 GCaMP variant acid residues imaging intracellular calcium transients GECI cell culture GCaMP Genetically GCaMP reagents oxygen environment photoconversion efficiency intracellular calcium concentrations calcium responsiveness calcium indicators