GCaMP3 is converted from green to red fluorescence after exposure to blue light.
Minrong Ai
Holly Mills
Makoto Kanai
Jason Lai
Jingjing Deng
Eric Schreiter
Loren Looger
Thomas Neubert
Greg Suh
10.1371/journal.pone.0138127.g001
https://plos.figshare.com/articles/figure/_GCaMP3_is_converted_from_green_to_red_fluorescence_after_exposure_to_blue_light_/1549243
<p><b>(A-B)</b> Fluorescent micrographs of dissected <i>Drosophila</i> brains expressing UAS-GCaMP3; <i>IR8a</i>-GAL4 <b>(A)</b> or UAS-GCaMP6s; NP225-GAL4 <b>(B)</b>. The dissected brains were exposed to blue light (mercury arc light passed through a Zeiss 63x oil-immersion objective) for different durations as indicated at the top of each panel. Green (top) and red (bottom) fluorescence micrographs were captured using a confocal microscope. Scale bar: 20μm. <b>(C)</b> Green-to-red photoconversion of GCaMP3 as quantified by the ratio of red-to-green fluorescence intensity (y-axis) following exposure to light from a Xenon lamp (x-axis).</p>
2015-09-18 03:49:16
GCaMP variant
acid residues
imaging intracellular calcium transients
GECI
cell culture
GCaMP Genetically
GCaMP reagents
oxygen environment
photoconversion efficiency
intracellular calcium concentrations
calcium responsiveness
calcium indicators