TY - DATA T1 - Data from: AFRICAN SWINE FEVER VIRUS ASSEMBLES A SINGLE MEMBRANE DERIVED FROM RUPTURE OF THE ENDOPLASMIC RETICULUM PY - 2016/07/14 AU - Dr. Cristina Suarez AU - Dr. German Andres AU - Mrs. Androniki Kolovou AU - Mrs. Simone Hoppe AU - Prof. Maria Luisa Salas AU - Dr. Paul Walther AU - Prof. Jacomine Krijnse Locker UR - https://wiley.figshare.com/articles/dataset/AFRICAN_SWINE_FEVER_VIRUS_ASSEMBLES_A_SINGLE_MEMBRANE_DERIVED_FROM_RUPTURE_OF_THE_ENDOPLASMIC_RETICULUM/1547921 DO - 10.6084/m9.figshare.1547921.v2 L4 - https://ndownloader.figshare.com/files/2270032 L4 - https://ndownloader.figshare.com/files/2270033 L4 - https://ndownloader.figshare.com/files/2270035 L4 - https://ndownloader.figshare.com/files/2270034 L4 - https://ndownloader.figshare.com/files/2270036 L4 - https://ndownloader.figshare.com/files/2270042 L4 - https://ndownloader.figshare.com/files/2270037 L4 - https://ndownloader.figshare.com/files/2270038 L4 - https://ndownloader.figshare.com/files/2270040 L4 - https://ndownloader.figshare.com/files/2270039 L4 - https://ndownloader.figshare.com/files/2270041 L4 - https://ndownloader.figshare.com/files/2270044 L4 - https://ndownloader.figshare.com/files/2270047 L4 - https://ndownloader.figshare.com/files/2270043 L4 - https://ndownloader.figshare.com/files/2270045 L4 - https://ndownloader.figshare.com/files/2270046 L4 - https://ndownloader.figshare.com/files/2270049 L4 - https://ndownloader.figshare.com/files/2270050 L4 - https://ndownloader.figshare.com/files/2270048 KW - membrane KW - virus KW - electron microscopy KW - tomography KW - asfv KW - Cell Biology KW - Microbiology N2 - Collective evidence argues that two members of the Nucleo-cytoplasmic large DNA viruses (NCLDVs) acquire their membrane from open membrane intermediates, postulated to be derived from membrane rupture. We now study membrane acquisition of the NCLDV African swine fever virus (ASFV). By electron tomography (ET) the virion assembles a single bilayer derived open membrane precursors that collect as ribbons in the cytoplasm. Biochemically, lumenal ER proteins are released into the cytosol, arguing that the open intermediates are ruptured ER membranes. ET shows that viral capsid assembles on the convex side of the open viral membrane to shape it into an icosahedron. The viral capsid is composed of tiny spikes with a diameter of ~5nm, connected to the membrane by a 6nm wide structure displaying thin striations, as assessed by several complementary EM imaging methods. Immature particles display an opening that closes after uptake of the viral genome and core proteins, followed by the formation of the mature virion. Together with our previous data this study shows a common principle of NCLDVs to build a single internal envelope from open membrane intermediates. Our data now provide biochemical evidence that these open intermediates result from rupture of a cellular membrane, the ER. ER -