TY - DATA T1 - Quantitative PCR of mutant and wildtype hypertrophic zones. PY - 2015/09/15 AU - Trevor L. Cameron AU - Katrina M. Bell AU - Irma L. Gresshoff AU - Lisa Sampurno AU - Lorna Mullan AU - Joerg Ermann AU - Laurie H. Glimcher AU - Raymond P. Boot-Handford AU - John F. Bateman UR - https://plos.figshare.com/articles/figure/_Quantitative_PCR_of_mutant_and_wildtype_hypertrophic_zones_/1545161 DO - 10.1371/journal.pgen.1005505.g004 L4 - https://ndownloader.figshare.com/files/2266342 KW - Disease Schmid metaphyseal chondrodysplasia KW - canonical ER stress sensors KW - XBP KW - exogenous misfolding protein KW - chop KW - perk KW - hypertrophic zone collagen KW - GADD KW - chondrocyte differentiation KW - hypertrophic chondrocytes KW - upr KW - atf KW - Col 10a Mouse models phenocopying MCDS KW - pathology KW - ColXN 617K KW - growth plate cartilage hypertrophic zone expansion KW - hypertrophic zone cartilage KW - hypertrophic zone expansion KW - ire KW - runx N2 - qPCR with primers specific for (A) Agc1, (B) Ctgf, (C) Matn1, (D) Creld2, (E) Derl3, (F) Ero1l, (G) Fgf21, (H) Steap1, and (I) p58IPK on cDNA derived from Wt, Xbp1CartΔEx2, ColXN617K and C/X hypertrophic zone aRNA. Plots depict mean fold differences with standard deviation from the mean, N = 3, statistical significance was determined using Student’s t test, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ER -