%0 Figure %A Nalluri, Srilatha %A Ghoshal-Gupta, Sampa %A Kutiyanawalla, Ammar %A Gayatri, Sitaram %A Rho Lee, Byung %A Jiwani, Shahanawaz %A M. Rojiani, Amyn %A V. Rojiani, Mumtaz %D 2015 %T (a): Interdependency of TIMP-1 and Bcl-2 proteins: H2009 cells and its clones were treated with 0.5μM Staurosporine (S), which showed a reduction of TIMP-1 protein expression. Treatment with a BH3 mimetic ABT-737(A) reduced TIMP-1 protein levels compared to the controls. Combined treatment with ABT-737 and Staurosporine (AS) showed a marked reduction of TIMP-1 expression, without any changes in TIMP-2. (b) TIMP-1 mRNA expression level after treatment with Staurosporine and ABT-737: HB1 and HB6 TIMP-1 levels were reduced with combined ABT-737 and Staurosporine treatment. Two Way Repeated Measure ANOVA showed p = 0.0063, indicating a very significant down regulation of relative expression of TIMP1 in the overexpressed clones after ABT+Staurosporine treatment indicating down-regulating Bcl2 affects TIMP1 level. (c) Co-immunoprecipitation of TIMP-1 and Bcl-2: A possible interaction between the Bcl-2 and TIMP-1 revealed by co-immunoprecipitation. Cell extracts from H2009 and it’s clones wer %U https://plos.figshare.com/articles/figure/_a_Interdependency_of_TIMP_1_and_Bcl_2_proteins_H2009_cells_and_its_clones_were_treated_with_0_5_956_M_Staurosporine_S_which_showed_a_reduction_of_TIMP_1_protein_expression_Treatment_with_a_BH3_mimetic_ABT_737_A_reduced_TIMP_1_protein_levels_compared_to_t/1541916 %R 10.1371/journal.pone.0137673.g003 %2 https://ndownloader.figshare.com/files/2261861 %K MMP inhibition activity %K overexpressing H 2009 cells %K protein levels %K hb %K Overexpressing TIMP %K cell lysates %K exhibit properties %K Inhibits Apoptosis %K TIMP 1 overexpressing clones %K PARP cleavage %K tumor kinetics %K T 2G %K Bax levels %K H 2009 lung adenocarcinoma cells %K lung cancer cell lines %K staurosporine treatment %K bad %K Cancer Biomarker %K rna %K p 90RSK %K lung adenocarcinoma cell line H 2009 %K bcl %K cns %K mitochondrial permeability %K lung adenocarcinoma cells %X

(a): Interdependency of TIMP-1 and Bcl-2 proteins: H2009 cells and its clones were treated with 0.5μM Staurosporine (S), which showed a reduction of TIMP-1 protein expression. Treatment with a BH3 mimetic ABT-737(A) reduced TIMP-1 protein levels compared to the controls. Combined treatment with ABT-737 and Staurosporine (AS) showed a marked reduction of TIMP-1 expression, without any changes in TIMP-2. (b) TIMP-1 mRNA expression level after treatment with Staurosporine and ABT-737: HB1 and HB6 TIMP-1 levels were reduced with combined ABT-737 and Staurosporine treatment. Two Way Repeated Measure ANOVA showed p = 0.0063, indicating a very significant down regulation of relative expression of TIMP1 in the overexpressed clones after ABT+Staurosporine treatment indicating down-regulating Bcl2 affects TIMP1 level. (c) Co-immunoprecipitation of TIMP-1 and Bcl-2: A possible interaction between the Bcl-2 and TIMP-1 revealed by co-immunoprecipitation. Cell extracts from H2009 and it’s clones were immunoprecipitated (IP) with anti-Bcl-2 or normal rabbit IgG as a negative control. The immunoblot (IB) was probed for TIMP-1, TIMP-2 and Bcl-2. No TIMP-2 was pulled down with Bcl-2, showing that only TIMP-1 is associated with Bcl-2 when apoptosis is induced. Input represents 60μg of cell extract.

%I PLOS ONE