TY - DATA T1 - PrPSc in N2a-FK cells is markedly degraded by the autophagy pathway. PY - 2015/09/14 AU - Daisuke Ishibashi AU - Takujiro Homma AU - Takehiro Nakagaki AU - Takayuki Fuse AU - Kazunori Sano AU - Hanae Takatsuki AU - Ryuichiro Atarashi AU - Noriyuki Nishida UR - https://plos.figshare.com/articles/figure/_PrP_Sc_in_N2a_FK_cells_is_markedly_degraded_by_the_autophagy_pathway_/1541292 DO - 10.1371/journal.pone.0137958.g002 L4 - https://ndownloader.figshare.com/files/2261084 KW - macroautophagy inducer rapamycin KW - fk KW - cells stably KW - Prion Strains KW - Chandler strain KW - 22 L KW - Infected Neuronal Cells Prion diseases KW - Host cells KW - neurodegenerative disorders KW - signal transduction pathways KW - PrPSc degradation KW - Abnormally Folded Prion Protein Degradation KW - 3 MA KW - prion protein KW - prion strain Fukuoka KW - accumulation N2 - Persistently prion-infected cells were treated with 1 to 10 mM of 3-methyladenine (3MA) and 0.2 to 1 μM rapamycin (Rap) for 48 h. Proteinase K (PK)-treated N2a-FK, -22L and-Ch cells, which vary in the prion strains, were loaded at concentrations of 100, 60 and 35 μg protein per lane onto a 15% polyacrylamide gels and subjected to SDS-PAGE. PrPSc was detected by western blotting using an anti-PrP antibody. For densitometric analysis, the images were scanned and the intensity of each band on the western blotting was quantified with respect to PrPSc expression levels in drug-treated prion-infected cells, respectively. The results are representative of at least three independent experiments, with each experiment performed in triplicate. *p < 0.05 and **p < 0.01 (one-way ANOVA followed by Tukey's test). ER -