Confirmation of HTS candidates by FP competition assay.
Xinlu Li
Marie Ménade
Guennadi Kozlov
Zheping Hu
Zheng Dai
Peter S. McPherson
Bernard Brais
Kalle Gehring
10.1371/journal.pone.0137298.g005
https://plos.figshare.com/articles/figure/_Confirmation_of_HTS_candidates_by_FP_competition_assay_/1541283
<p>A and B. 5 nM F12-ATP and 200 nM GST-HEPN were titrated with the hits to confirm their binding to GST-HEPN. C. Affinities of the compounds for HEPN were estimated from the competition assays and summarized along with their chemical structures.</p>
2015-09-14 02:46:08
cell culture studies
neuron
binding
neurodegenerative disease autosomal recessive spastic ataxia
ARSACS
sacsin
protein quality control
nucleotide
HEPN domain
FP competition assays