TY - DATA T1 - Single nucleotide polymorphisms of TLR’s. PY - 2015/09/11 AU - Charles S. Berenson AU - Ragina L. Kruzel AU - Catherine T. Wrona AU - Manoj J. Mammen AU - Sanjay Sethi UR - https://plos.figshare.com/articles/figure/_Single_nucleotide_polymorphisms_of_TLR_8217_s_/1540571 DO - 10.1371/journal.pone.0134209.g001 L4 - https://ndownloader.figshare.com/files/2259389 KW - 1237C KW - snp KW - Impaired Innate COPD Alveolar Macrophage Responses KW - alveolar macrophage responses KW - nontypeable H KW - dna KW - polymerase chain reaction KW - fev KW - nucleotide polymorphism expression KW - nucleotide polymorphisms KW - tlr KW - COPD alveolar macrophage responses KW - il KW - nontypeable Haemophilus influenzae N2 - PCR-based RFLP’s were performed with genomic DNA from each participant. Representative agarose gels of shown for: TLR9 (T1237C) (Panel A), TLR9 (T1486C) (Panel B), TLR4 (Thr399Ile) (Panel C), TLR4 (Asp299Gly) (Panel D) and TLR2 (Arg753Gln) (Panel E). Numbers in left margin of each gel correspond to DNA ladder base pairs. Lanes with restriction fragments for heterozygotes and homozygotes of each SNP are distinguishable from restriction fragments for wildtypes. Representative patient samples are shown in each lane of each panel. ER -