Molecular sensors of intracellular calcium regulate PspA induced PD-L1 expression. Mohit Vashishta Naeem Khan Subhash Mehto Devinder Sehgal Krishnamurthy Natarajan 10.1371/journal.pone.0133601.g005 https://plos.figshare.com/articles/figure/_Molecular_sensors_of_intracellular_calcium_regulate_PspA_induced_PD_L1_expression_/1494996 <p>For Panel A, mouse bone marrow derived DCs were transfected with siRNAs against indicated molecules for 36h, followed by stimulation with 15 μg/ml PspA for 24h. PD-L1 levels were monitored by flow cytometry. Dotted lines represent unstimulated cells transfected with control siRNAs. Thin lines represent cells transfected with control siRNAs followed by stimulations with 15 μg/ml PspA. Bold lines represent cells transfected with siRNAs specific to the indicated molecules followed by stimulation with 15 μg/ml PspA. Data from one of three independent experiments is shown. In Panel B, PD-L1 expression is represented as bars indicating fold increase in Relative Mean Fluorescence Intensity (MFI) for various groups. In panel B, bars represent mean ± SD of three independent experiments. ‘ns’ represents non-significant differences between compared groups.</p> 2015-07-27 02:53:36 surface antigen Pneumococcal surface protein pspa ros Calcium Dependent Manner Pneumonia expression TLR 2 Regulates Programmed Death Ligand 1 Expression Streptococcus pneumoniae infection dc Pneumococal Surface Protein